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Titolo:
GLUTAMINE TRANSPORT IN MOUSE CEREBRAL ASTROCYTES
Autore:
NAGARAJA TN; BROOKES N;
Indirizzi:
UNIV MARYLAND,SCH MED,DEPT PHARMACOL & EXPTL THERAPEUT,655 W BALTIMORE ST BALTIMORE MD 21201 UNIV MARYLAND,SCH MED,DEPT PHARMACOL & EXPTL THERAPEUT BALTIMORE MD 21201
Titolo Testata:
Journal of neurochemistry
fascicolo: 4, volume: 66, anno: 1996,
pagine: 1665 - 1674
SICI:
0022-3042(1996)66:4<1665:GTIMCA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-TRANSPORT; CULTURED ASTROCYTES; RAT-LIVER; SYNAPTOSOMES; SYSTEMS; CELLS; ASPARAGINE; CYCLE; PH;
Keywords:
SYSTEM A; SYSTEM ASC; SYSTEM L; SYSTEM N; LITHIUM; PH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
T.N. Nagaraja e N. Brookes, "GLUTAMINE TRANSPORT IN MOUSE CEREBRAL ASTROCYTES", Journal of neurochemistry, 66(4), 1996, pp. 1665-1674

Abstract

We measured initial influx and exchange of [C-14]glutamine in primaryastrocyte cultures in the presence and absence of Na+. Kinetic analysis of transport in Na+-free solution indicated two saturable Na+-independent components, one of which was identifiable functionally as system L(1) transport. In the presence of Na+, multiple hyperbolic components were not resolvable from the kinetic data. Nevertheless, other evidence supported participation by at least three Na+-dependent neutral amino acid transporters (systems A, ASC, and N). System A transport of glutamine was usually absent or minimal, based on lack of inhibition by alpha-(methylamino)isobutyric acid. However, vigorous system A-mediated transport emerged after derepression by substrate deprivation. Participation by system ASC was indicated by trans-acceleration of Na+-dependent uptake, preferential inhibition of an Li+-intolerant componentof uptake by cysteine, and inhibition by cysteine of a component resistant to inhibition by histidine and alpha-(methylamino) isobutyric acid. Because nonsaturable transport of glutamine appeared negligible, and system L transport of glutamine was suppressed in the presence of Na+, low-affinity system ASC transport may be the major route of exportof glutamine from astrocytes. At 700 mu M glutamine, the primary uptake route was system N transport, identified on the basis of selective inhibition by histidine and asparagine, pH sensitivity, and tolerance of Li+ in place of Na+.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 06:29:33