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Titolo:
INWARDLY RECTIFYING K-CELLS FROM GUINEA-PIG HEART( CHANNELS IN FRESHLY DISSOCIATED CORONARY ENDOTHELIAL)
Autore:
VONBECKERATH N; DITTRICH M; KLIEBER HG; DAUT J;
Indirizzi:
UNIV MARBURG,INST NORMALE & PATHOL PHYSIOL,DEUTSCHHAUSSTR 2 D-35033 MARBURG GERMANY UNIV MARBURG,INST NORMALE & PATHOL PHYSIOL D-35033 MARBURG GERMANY
Titolo Testata:
Journal of physiology
fascicolo: 2, volume: 491, anno: 1996,
pagine: 357 - 365
SICI:
0022-3751(1996)491:2<357:IRKFGH>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; ACTIVATED POTASSIUM; CYTOSOLIC CALCIUM; IONIC CURRENTS; RECTIFIER; RECTIFICATION; BRADYKININ; EXPRESSION; BARIUM; MG-2+;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
N. Vonbeckerath et al., "INWARDLY RECTIFYING K-CELLS FROM GUINEA-PIG HEART( CHANNELS IN FRESHLY DISSOCIATED CORONARY ENDOTHELIAL)", Journal of physiology, 491(2), 1996, pp. 357-365

Abstract

1. Inwardly rectifying K+ (I-K(IR)) currents of freshly dissociated coronary endothelial cells from guinea-pig heart were investigated withthe perforated-patch technique. 2. The whole-cell current-voltage relationship of endothelial cells showed strong inward rectification. Increasing the extracellular K+ resulted in an increase of inward currents. The slope conductance of the cells in the potential range negative to the calculated potassium equilibrium potential (E(K)) with 5, 60 and 150 mM external potassium was 0.18 +/- 0.14, 0.55 +/- 0.50 and 0.63 /- 0.29 nS (mean +/- S.D.), respectively. 3. To quantify the steepness of inward rectification, the voltage dependence of the chord conductance of the cells was fitted with a Boltzmann function. The slope factor k describing the steepness of the relationship was 6.8 +/- 1.5 mV. 4. Extracellular barium induced a potential- and time-dependent block of inward currents through endothelial K-IR channels. Half-maximum inhibition of I-K(IR) currents was achieved with less than or equal to 1 mu M barium at a membrane potential of - 70 mV in a solution containing 60 mM K+. 5. Whole-cell inward currents revealed the opening and closing of single K-IR channels. The single-channel conductance was 26 +/- 3 pS with 60 mM external K+ and 33 +/- 6 pS with 150 mM external K+.6. Our results suggest that the electrical properties of freshly dissociated endothelial cells are to a large extent determined by five to sixty active strong inwardly rectifying K+ (K-IR) channels.

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Documento generato il 02/12/20 alle ore 17:02:00