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Titolo:
PARACRINE AUTOCRINE REGULATION OF PANCREATIC LET CELL-PROLIFERATION AND DIFFERENTIATION IN THE HAMSTER - STUDIES USING PARABIOSIS/
Autore:
ROSENBERG L; KAHLENBERG M; VINIK AI; DUGUID WP;
Indirizzi:
MONTREAL GEN HOSP,DEPT SURG,1650 CEDAR AVE,ROOM L9-424 MONTREAL PQ H3G 1A4 CANADA MONTREAL GEN HOSP,DEPT PATHOL MONTREAL PQ H3G 1A4 CANADA MCGILL UNIV MONTREAL PQ CANADA EASTERN VIRGINIA MED SCH,INST DIABET NORFOLK VA 23501
Titolo Testata:
Clinical and investigative medicine
fascicolo: 1, volume: 19, anno: 1996,
pagine: 3 - 12
SICI:
0147-958X(1996)19:1<3:PAROPL>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH FACTOR-I; HUMAN-FETAL PANCREAS; MONOLAYER-CULTURES; SOMATOMEDIN-C; INSULIN; INDUCTION; NESIDIOBLASTOSIS; HORMONE; REPLICATION; ADULT;
Keywords:
PANCREAS; HAMSTER; ISLET CELL; DIFFERENTIATION; PARABIOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
L. Rosenberg et al., "PARACRINE AUTOCRINE REGULATION OF PANCREATIC LET CELL-PROLIFERATION AND DIFFERENTIATION IN THE HAMSTER - STUDIES USING PARABIOSIS/", Clinical and investigative medicine, 19(1), 1996, pp. 3-12

Abstract

Partial obstruction of the pancreatic duct in hamsters leads to new islet formation and reversal of streptozotocin diabetes. The purpose ofthis study was to delineate the mechanism by which endocrine cell proliferation and differentiation is mediated in this model. Six pairs ofparabiotic hamsters were established and partial duct obstruction wasinducted in 1 parabiont from each pair. At 6 weeks, the pancreatic weight (mg/100g bw); DNA (mu g/100g bw) and protein content (mg/100g bw)showed 28% (167 +/- 21 vs. 130 +/- 17), 32% (1,052 +/- 206 vs. 795 +/- 159), and 20% (25.4 +/- 6.6 vs. 21.2 +/- 1.9) increases (p < 0.05), respectively, over the non-wrapped parabionts. Morphometric analysis demonstrated the presence of new islets in the wrapped pancreata with a100% increase in the number of islets/mm(2) compared with non-wrappedcontrols (0.90 +/- 0.5 vs. 1.8 +/- 0.7, p < 0.01). A cytosol extract was prepared from duct-obstructed pancreases, and 4 mu l/g bw injectedi.p. twice daily for 2 d produced significant increases in pancreaticweight and DNA content of 12% and 40%, respectively. Cytosol extract from non-wrapped pancreata had no effects compared with saline. When wrapped cytosol extract was injected for 21 d, the labeling index of ductular and islet cells (% cell nuclei labeled with H-3-TdR) was increased 10- and 6-fold respectively over controls (2.42 +/- 0.28 vs. 0.23 /- 0.01 and 1.17 +/- 0.01 vs. 0.25 +/- 0.04, respectively, p < 0.01). The trophic effects observed in this model of islet cell proliferation and differentiation did not appear to be mediated by a humoral mechanism because the changes induced by partial obstruction were not observed in the non-operated parabiont. Control of pancreatic endocrine cell growth in this model appears to involve paracrine and/or autocrine regulatory mechanisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 14:36:37