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Titolo:
DENDRITIC MORPHOLOGY OF CARDIAC-RELATED MEDULLARY NEURONS DEFINED BY CIRCUIT-SPECIFIC INFECTION BY A RECOMBINANT PSEUDORABIES VIRUS EXPRESSING BETA-GALACTOSIDASE
Autore:
STANDISH A; ENQUIST LW; MISELIS RR; SCHWABER JS;
Indirizzi:
DUPONT CO INC WILMINGTON DE 19880 PRINCETON UNIV,DEPT MOLEC BIOL PRINCETON NJ 08544 UNIV PENN PHILADELPHIA PA 19104
Titolo Testata:
Journal of neurovirology
fascicolo: 5-6, volume: 1, anno: 1995,
pagine: 359 - 368
SICI:
1355-0284(1995)1:5-6<359:DMOCMN>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; PREGANGLIONIC NEURONS; RAT-HEART; ORGANIZATION; NUCLEUS; VAGUS;
Keywords:
DENDRITIC MORPHOLOGY; DENDRITES; RAT HEART; CARDIAC CIRCUIT; VIRAL TRACING; PSEUDORABIES VIRUS; BETA-GALACTOSIDASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
A. Standish et al., "DENDRITIC MORPHOLOGY OF CARDIAC-RELATED MEDULLARY NEURONS DEFINED BY CIRCUIT-SPECIFIC INFECTION BY A RECOMBINANT PSEUDORABIES VIRUS EXPRESSING BETA-GALACTOSIDASE", Journal of neurovirology, 1(5-6), 1995, pp. 359-368

Abstract

The transneuronal herpesvirus tracer, pseudorabies virus (PRV) was used to determine the dendritic architecture of cardiac-related neurons. We constructed a derivative of the Bartha strain of PRV called PRV-BaBlu, that carries the lacZ gene of E. coli. Expression of Beta-galactosidase by this recombinant virus enabled us to define the dendritic morphology of motoneurons and interneurons that innervate the heart. beta-galactosidase antigen filled dendritic processes that were clearly revealed by antibodies to beta-galactosidase. In contrast, the standardenzymatic reaction for detection of beta-galactosidase activity stained the cell soma well, but was inferior for labeling dendrites. Following PRV-BaBlu cardiac injection, infected neurons were clearly definedand labeled dendrites could be traced for long distances, sometimes greater than 800 mu m from the cell body. Labeled dendrites of cardiomotor neurons primarily located in the nucleus ambiguus (NA) were extensive and sometimes intertwined with dendrites from other labeled motoneurons, Dendrites of labeled neurons in the dorsal motor nucleus of thevagus (DMV) typically extended in the mediolateral direction in the transverse plane. Transynaptically labeled interneurons interposed between the cardiorespiratory region of the nucleus fil-actus solitarius (NTS) and the NA were primarily located in the NA region and the reticular are, the area between the DMV and NA. These interneurons had long dendrites extending along the reticular are in the transverse plane. The dendritic arborizations of infected cardiac-related neurons in the NTS were variable in extent. We conclude that antibody detection of beta-galactosidase expressed by PRV-BaBlu after infection of neural cardiac circuits provides a superior method to define the dendrites and dendritic fields of cardiac-related motoneurons and interneurons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 21:05:38