Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
STUDIES OF LIVER REPOPULATION USING THE DIPEPTIDYL PEPTIDASE IV-DEFICIENT RAT AND OTHER RODENT RECIPIENTS - CELL-SIZE AND STRUCTURE RELATIONSHIPS REGULATE CAPACITY FOR INCREASED TRANSPLANTED HEPATOCYTE MASS INTHE LIVER LOBULE
Autore:
RAJVANSHI P; KERR A; BHARGAVA KK; BURK RD; GUPTA S;
Indirizzi:
YESHIVA UNIV ALBERT EINSTEIN COLL MED,LONG ISL JEWISH MED CTR,DEPT MED BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,LONG ISL JEWISH MED CTR,DEPT MED BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,LONG ISL JEWISH MED CTR,DEPT RADIOL BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,LONG ISL JEWISH MED CTR,DEPT PEDIAT BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,LONG ISL JEWISH MED CTR,DEPT MICROBIOL & IMMUNOL BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,LONG ISL JEWISH MED CTR,MARION BESSIN LIVER RES CTR,GI DIV BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,LONG ISL JEWISH MED CTR,DEPT OBSTET & GYNECOL BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,LONG ISL JEWISH MED CTR,DEPT NUCL MED BRONX NY 10461
Titolo Testata:
Hepatology
fascicolo: 3, volume: 23, anno: 1996,
pagine: 482 - 496
SICI:
0270-9139(1996)23:3<482:SOLRUT>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXVIVO GENE-THERAPY; SURFACE-ANTIGEN; MESSENGER-RNA; ALBUMIN; ENGRAFTMENT; EXPRESSION; RETICULUM; SYSTEM; SITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
P. Rajvanshi et al., "STUDIES OF LIVER REPOPULATION USING THE DIPEPTIDYL PEPTIDASE IV-DEFICIENT RAT AND OTHER RODENT RECIPIENTS - CELL-SIZE AND STRUCTURE RELATIONSHIPS REGULATE CAPACITY FOR INCREASED TRANSPLANTED HEPATOCYTE MASS INTHE LIVER LOBULE", Hepatology, 23(3), 1996, pp. 482-496

Abstract

The feasibility of liver repopulation with hepatocytes has been shown, although clinical applications demand significant hepatic replacement. To show whether portal vascular bed in large animals could accomodate a greater cell number, we analyzed Liver repopulation in syngeneic Fischer 344 rats deficient in dipeptidyl peptidase IV. This system allowed localization of transplanted normal hepatocytes in liver or various ectopic sites, as well as dual studies for analysis of gene expression. Interestingly, the product of a dipeptidyl peptidase IV substrateinactivated bile canalicular adenosine triphosphatase (ATPase) activity in normal but not in dipeptidyl peptidase IV-deficient rats, which allowed localization of dipeptidyl peptidase IV-deficient hepatocytes in normal rat liver for additional reversed transplantation systems. Further studies with genetically marked cells showed that because of the size difference between hepatocytes and portal vein radicles, intrasplenically transplanted cells were distributed in periportal areas (zone 1) in mice, whereas in larger animals (rats or rabbits) cells were also distributed downstream to midlobular (zone 2) or perivenous (zone3) areas. Transplantation of an escalating number of hepatocytes showed that adult rats tolerated intrasplenic injection of a large cell number in single sessions (up to 1 x 10(8), similar to 10% to 15% of thehost hepatocyte mass). Morphometric analysis of recipient livers showed survival of a significantly greater cell number with incorporation in host liver plates. At 4 weeks, transplantation of 2 x 10(7) hepatocytes into adult rats led to survival of 1.4 +/- 1.0 x 10(6) transplanted cells/cm(3) liver, whereas after transplantation of 5 x 10(7) cellsor 7.5 x 10(7) cells, the number of surviving transplanted cells in the liver significantly increased to 4.1 +/- 1.4 x 10(6) transplanted cells/cm(3) liver (mean, 2.9-fold; P < .003) and 5.5 +/- 1.3 X 10(6) transplanted cells/cm(3) liver (mean, 3.9-fold; P < .003), respectively. When cells were injected in greater numbers, transplanted hepato cytes retained normal function and produced more serum albumin or hepatitis B surface antigen in deficient hosts. These data indicate the feasibility in larger animals of significant liver repopulation with hepatocyte transplantation. Use of dipeptidyl peptidase N-deficient rats should help further analysis of mechanisms in liver repopulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/10/20 alle ore 09:04:17