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Titolo:
CD44 CHONDROITIN SULFATE PROTEOGLYCAN AND ALPHA-2-BETA-1 INTEGRIN MEDIATE HUMAN-MELANOMA CELL-MIGRATION ON TYPE-IV COLLAGEN AND INVASION OFBASEMENT-MEMBRANES/
Autore:
KNUTSON JR; IIDA J; FIELDS GB; MCCARTHY JB;
Indirizzi:
UNIV MINNESOTA,DEPT LAB MED & PATHOL MINNEAPOLIS MN 55455 UNIV MINNESOTA,DEPT LAB MED & PATHOL MINNEAPOLIS MN 55455 UNIV MINNESOTA,CTR BIOCHEM ENGN MINNEAPOLIS MN 55455
Titolo Testata:
Molecular biology of the cell
fascicolo: 3, volume: 7, anno: 1996,
pagine: 383 - 396
SICI:
1059-1524(1996)7:3<383:CCSPAA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR-MATRIX; MONOCLONAL-ANTIBODY; CYTOPLASMIC DOMAINS; PROTEASE INHIBITORS; SYNTHETIC PEPTIDES; HIGH ENDOTHELIUM; CARCINOMA-CELLS; VI COLLAGEN; ADHESION; LAMININ;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
85
Recensione:
Indirizzi per estratti:
Citazione:
J.R. Knutson et al., "CD44 CHONDROITIN SULFATE PROTEOGLYCAN AND ALPHA-2-BETA-1 INTEGRIN MEDIATE HUMAN-MELANOMA CELL-MIGRATION ON TYPE-IV COLLAGEN AND INVASION OFBASEMENT-MEMBRANES/", Molecular biology of the cell, 7(3), 1996, pp. 383-396

Abstract

Tumor cell invasion of basement membranes (BM) represents one of the critical steps in the metastatic process. Tumor cell recognition of individual BM matrix components may involve individual cell adhesion receptors, such as integrins or cell surface proteoglycans, or may involve a coordinate action of both types of receptors. In this study, we have focused on the identification of a cell surface CD44/chondroitin sulfate proteoglycan (CSPG) and alpha 2 beta 1 integrin on human melanoma cells that are both directly involved in the in vitro invasion of reconstituted BM via a type IV collagen-dependent mechanism. Interferingwith cell surface expression of human melanoma CSPG with either rho-nitrophenyl-beta-D-xylopyranoside treatment or anti-CD44 monoclonal antibody (mAb) preincubation inhibits melanoma cell invasion through reconstituted BM. These treatments also strongly inhibit melanoma cell migration on type IV collagen, however, they are ineffective at inhibiting cell adhesion to type IV collagen. Purified melanoma cell surface CD44/CSPG, or purified chondroitin sulfate, bind to type IV collagen affinity columns, consistent with a role for CD44/CSPG-type TV collagen interactions in mediating tumor cell invasion. In contrast, melanoma cell migration on laminin (LM) does not involve CD44/CSPG, nor does CD44/CSPG bind to LM, suggesting that CD44/CSPG-type IV collagen interactions are specific in nature. Additionally, anti-alpha 2 and anti-beta 1integrin mAbs are capable of blocking melanoma cell invasion of reconstituted BM. Both of these anti-integrin mAbs inhibit melanoma cell adhesion and migration on type TV collagen, whereas only anti-beta 1 mAbinhibits cell adhesion to LM. Collectively, these results indicate that melanoma cell adhesion to type IV collagen is an important consideration in invasion of reconstituted BM in vitro, and suggest that CD44/CSPG and alpha 2 beta 1 integrin may collaborate to promote human melanoma cell adhesion, migration, and invasion in vivo.

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Documento generato il 30/11/20 alle ore 07:08:04