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Titolo:
INDUCTION OF SIV CAPSID-SPECIFIC CTL AND MUCOSAL SIGA IN MICE IMMUNIZED WITH A RECOMBINANT SALMONELLA-TYPHIMURIUM AROA MUTANT
Autore:
VALENTINE PJ; MEYER K; RIVERA MM; LIPPS C; PAUZA D; MAZIARZ RT; SO M; HEFFRON F;
Indirizzi:
OREGON HLTH SCI UNIV,DEPT MICROBIOL & IMMUNOL,3181 SW SAM JACKSON PK RD PORTLAND OR 97201 OREGON HLTH SCI UNIV,DEPT MICROBIOL & IMMUNOL PORTLAND OR 97201 UNIV WISCONSIN,SCH MED,DEPT PATHOL MADISON WI 53706 UNIV WISCONSIN,SCH MED,DEPT LAB MED MADISON WI 53706 OREGON HLTH SCI UNIV,DIV HEMATOL & MED ONCOL PORTLAND OR 97201 VET ADM MED CTR PORTLAND OR 97201
Titolo Testata:
Vaccine
fascicolo: 2, volume: 14, anno: 1996,
pagine: 138 - 146
SICI:
0264-410X(1996)14:2<138:IOSCCA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIVE ATTENUATED SALMONELLA; FOREIGN ANTIGENS; VACCINE STRAINS; PROTEINS; EXPRESSION; VECTOR; CONSTRUCTION; CARRIERS; SYSTEM; GENES;
Keywords:
SALMONELLA; VACCINE; GROEL PROMOTER; CYTOTOXIC T-LYMPHOCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
P.J. Valentine et al., "INDUCTION OF SIV CAPSID-SPECIFIC CTL AND MUCOSAL SIGA IN MICE IMMUNIZED WITH A RECOMBINANT SALMONELLA-TYPHIMURIUM AROA MUTANT", Vaccine, 14(2), 1996, pp. 138-146

Abstract

We have developed a nerv expression system based on the E. coli growth promoter. The suicide vector constructed (called APC vector) allows simultaneous attenuation of a Salmonella strain by disruption of the coding sequence for aroA and stable integration of a gene into the bacterial chromosome High-level expression of antigen is achieved after Salmonella is taken tcp by macrophages, a major antigen processing cell of the host. The chloramphenicol acetyltransferase (CAT) and the simian immunodeficiency virus capsid (p27gag) genes were cloned downstream of the groEL promoter and expressed within S. typhimurium. By measuring CAT activity, we showed that the groEL promoter tl as up-regulated during infection of the J774 macrophage line. The immune response to SIV capsid was assessed in Balb/c mice given one oral dose of vaccine. Alocal mucosal secretory IgA response against SIV capsid was detected but no systemic antibody response to the same antigen. A systemic CTL response was detected as early as 28 days to as late as 70 days post-immunization. CTL activity was MHC restricted (H-2(d)) and was mediatedby CD3(+), CD8(+), CD4(-) T-lymphocytes. These results indicate that with only one oral dose of recombinant Salmonella using the APC vector, a systemic CTL response and a mucosal secretory response against theSIV capsid antigen are elicited in a mouse model.

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Documento generato il 31/10/20 alle ore 11:42:35