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Titolo:
RNASE-P FROM A PHOTOSYNTHETIC ORGANELLE CONTAINS AN RNA HOMOLOGOUS TOTHE CYANOBACTERIAL COUNTERPART
Autore:
BAUM M; CORDIER A; SCHON A;
Indirizzi:
UNIV WURZBURG,INST BIOCHEM,BIOZENTRUM,HUBLAND D-97074 WURZBURG GERMANY UNIV WURZBURG,INST BIOCHEM,BIOZENTRUM D-97074 WURZBURG GERMANY
Titolo Testata:
Journal of Molecular Biology
fascicolo: 1, volume: 257, anno: 1996,
pagine: 43 - 52
SICI:
0022-2836(1996)257:1<43:RFAPOC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBONUCLEASE-P; CYANOPHORA-PARADOXA; SCHIZOSACCHAROMYCES-POMBE; GREEN CHLOROPLASTS; PROTEIN; GENE; SUBUNIT; CYANELLE; IDENTIFICATION; MITOCHONDRIA;
Keywords:
CYANELLE; EVOLUTION; PRE-TRANSFER-RNA PROCESSING; RIBONUCLEASE P; RIBOZYME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
M. Baum et al., "RNASE-P FROM A PHOTOSYNTHETIC ORGANELLE CONTAINS AN RNA HOMOLOGOUS TOTHE CYANOBACTERIAL COUNTERPART", Journal of Molecular Biology, 257(1), 1996, pp. 43-52

Abstract

RNase P is a ribonucleoprotein enzyme in all organisms and organellesinvestigated so far, with the exception of chloroplasts where no enzyme-associated RNA has been detected to date. As an approach to answer the question whether an RNA component is present in RNase P from photosynthetic organelles, we have used a phylogenetically oriented strategy and searched for RNase P RNA in a postulated intermediate in plastidevolution, the cyanelle of Cyanophora paradoxa. We have detected a 351 nucleotide long RNA similar to cyanobacterial RNase P RNAs, with a proposed secondary structure that closely resembles a bacterial consensus. The RNA is encoded on the cyanelle genome and copurifies with enzyme activity The RNA is not catalytically active by itself, but the activity of the cyanelle RNase P holoenzyme is destroyed by nuclease treatment, indicating an essential role of the RNA. Hence cyanelle RNase Pcombining properties of bacterial and eukaryotic enzymes, occupies anintermediate position in RNA enzyme evolution. The first description of an RNA component in RNase P from a photosynthetic organelle might thus be an important step towards an understanding of plastid RNase P structure and function. (C) 1996 Academic Press Limited

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 11:29:29