Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EFFECT OF RH-GMCSF AND RH-GCSF ON OXYGEN-FREE RADICAL PRODUCTION BY HUMAN NEUTROPHILS AND BLOOD MONOCYTE-DERIVED HUMAN MACROPHAGES
Autore:
ALSHAMMARY FJ; ALZAMEL F; ELSHEWEMI S; SOLIMAN R;
Indirizzi:
KING SAUD UNIV,DEPT CLIN LAB SCI,COLL APPL MED SCI,POB 10219 RIYADH 11433 SAUDI ARABIA KING SAUD UNIV,DEPT CLIN LAB SCI,COLL APPL MED SCI RIYADH 11433 SAUDIARABIA
Titolo Testata:
Renal physiology and biochemistry
fascicolo: 6, volume: 18, anno: 1995,
pagine: 278 - 287
SICI:
1011-6524(1995)18:6<278:EORARO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; PROTEIN KINASE-C; GRANULOCYTE-MACROPHAGE; OXIDATIVE-METABOLISM; CYTOCHALASIN-B; CYTO-TOXICITY; NADPH OXIDASE; GM-CSF; CELLS; PHAGOCYTES;
Keywords:
OXYGEN FREE RADICALS; HUMAN NEUTROPHILS; HUMAN BLOOD MONOCYTE DERIVED MACROPHAGES; GRANULOCYTE-MACROPHAGE COLONY STIMULATING FACTOR; GRANULOCYTE COLONY STIMULATING FACTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
F.J. Alshammary et al., "EFFECT OF RH-GMCSF AND RH-GCSF ON OXYGEN-FREE RADICAL PRODUCTION BY HUMAN NEUTROPHILS AND BLOOD MONOCYTE-DERIVED HUMAN MACROPHAGES", Renal physiology and biochemistry, 18(6), 1995, pp. 278-287

Abstract

The in vitro effect of recombinant human granulocyte-macrophage colony stimulating factor (rh-GMCSF) and recombinant human granulocyte colony stimulating factor (rh-GCSF) on oxygen free radical (OFR) generation by human neutrophils and blood monocytes derived human macrophages stimulated with phorbol myristate acetate was investigated and compared. The production of OFR by neutrophils and macrophages was time dependent, and the maximum release of OFR by neutrophils and macrophages wasmeasured 90 and 180 min after stimulation with phorbol myristate acetate, respectively. The priming effects or rh-GMCSF and rh-GCSF on OFR production by human neutrophils and macrophages was dose dependent. The maximum generation of OFR by neutrophils occurred when primed with 1,000 U/ml of rh-GMCSF and reached 2.383+/-0.191 nmol/10(5):: neutrophils/90 min as compared with 1.072+/-0.113 nmol/10(5) neutrophils/90 minin the unprimed controls. This represents a 122.20% increase in OFR generation (p<0.001). However, the percentage of maximum increase in OFR production was 57.84 when neutrophils were primed with a concentration of 5,000 U of rh-GCSF/ml. In 72-hour-old human macrophages, much higher levels of OFR production as compared with neutrophils were measured following stimulation with phorbol myristate acetate. The maximum generation of OFR was measured in macrophages primed for 45 min with 500 U/ mi of rh-GMCSF. These cells produced 5.960+/-2.075 nmol/5 x 10(4)macrophage/180 min as compared with 4.563+/-1.773 nmol/5 x 10(4) unprimed macrophages/180 min (p<0.001). In macrophages primed with rh-GCSFhowever, the maximum OFR production was induced by a dose of 5,000 U/ml and reached 6.902+/-1.163 nmol/5 x 10(4) macrophages/180 min as compared with 4.563+/-1.773 nmol/5 x 10(4) macrophages/180 min in the unprimed controls (p<0.05). In conclusion, the priming effect of rh-GMCSFon OFR generation by human macrophages and neutrophils was more potent than that of rh-GCSF, both in the extent of augmentation and in the dose required to produce maximum OFR generation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/02/20 alle ore 18:12:55