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Titolo:
DIFFERENT PHARMACOLOGICAL CHARACTERISTICS IN L(6) AND C2C12 MUSCLE-CELLS AND INTACT RAT SKELETAL-MUSCLE FOR AMYLIN, CGRP AND CALCITONIN
Autore:
PITTNER RA; WOLFELOPEZ D; YOUNG AA; BEAUMONT K;
Indirizzi:
AMYLIN PHARMACEUT INC,9373 TOWNE CTR DR SAN DIEGO CA 92121
Titolo Testata:
British Journal of Pharmacology
fascicolo: 5, volume: 117, anno: 1996,
pagine: 847 - 852
SICI:
0007-1188(1996)117:5<847:DPCILA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-RELATED PEPTIDE; GLYCOGEN-PHOSPHORYLASE; L6 MYOCYTES; INSULIN; GLUCOSE; METABOLISM; RESISTANCE; MECHANISM; RECEPTORS; SYNTHASE;
Keywords:
AMYLIN; CGRP; CALCITONIN; PEPTIDE RECEPTORS; SKELETAL MUSCLE; L(6); GLUCOSE METABOLISM; CYCLIC AMP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
R.A. Pittner et al., "DIFFERENT PHARMACOLOGICAL CHARACTERISTICS IN L(6) AND C2C12 MUSCLE-CELLS AND INTACT RAT SKELETAL-MUSCLE FOR AMYLIN, CGRP AND CALCITONIN", British Journal of Pharmacology, 117(5), 1996, pp. 847-852

Abstract

1 We compared the ability of rat amylin, rat calcitonin gene-related peptide (CGRP) and rat and salmon calcitonins to elevate cyclic AMP levels and to inhibit [U-C-14]-glucose incorporation into glycogen in insulin-stimulated intact rat soleus muscle and in two cell lines derived from rodent skeletal muscle, L(6) and C2C12 2 In intact soleus muscle, both amylin (EC(50)s of 0.7-6.1 nM) and salmon calcitonin (EC(50)s Of 0.5-1.4 nM) were more potent than CGRP (EC(50)s of 5.6-15.8 nM) andwere much more potent than rat calcitonin (EC(50)s of 50-137 nM) at stimulating cyclic AMP production, activating glycogen phosphorylase and inhibiting insulin-stimulated [C-14]-glycogen formation. 3 In contrast, in both L(6) and C2C12 cells, CGRP (EC(50)s Of 0.042-0.12 nM) stimulated cyclic AMP formation and inhibited insulin-stimulated [U-C-14]-glucose incorporation into glycogen approximately 1000 times more potently than amylin (EC(50)s 34-240 nM), while salmon calcitonin was without measurable effect. 4 There was a correlation between elevation of cyclic AMP and inhibition of insulin-stimulated [U-C-14]-glucose incorporation into glycogen evoked by these peptides in both intact muscle (r(2)=0.69, P<0.0004) and muscle cell lines (r(2)=0.96, P<0.0001). 5 In conclusion, the effects of amylin, CGRP, and calcitonin on soleus muscle glycogen metabolism appear to be mediated by adenylyl cyclase-coupled receptors which show a pharmacological profile similar to high affinity amylin binding sites that have been previously reported in rat brain. In contrast, the effects of amylin and CGRP in L(6) and C2C12 rodent muscle cell lines appear to be mediated by adenylyl cyclase-coupled receptors that behave like CGRP receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:30:14