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Titolo:
OXYTOCIN RECEPTORS EXPRESSED AND COUPLED TO CA2-MUSCLE CELL-LINE( SIGNALING IN A HUMAN VASCULAR SMOOTH)
Autore:
YAZAWA H; HIRASAWA A; HORIE K; SAITA Y; IIDA E; HONDA K; TSUJIMOTO G;
Indirizzi:
YAMANOUCHI PHARMACEUT CO LTD,INST DRUG DISCOVERY RES,DRUD SERENDIP RES LABS,21 MIYUKIGAOKA TSUKUBA IBARAKI 305 JAPAN NATL CHILDRENS MED RES CTR,DEPT MOLEC CELL PHARMACOL,SETAGAYA KU TOKYO 154 JAPAN
Titolo Testata:
British Journal of Pharmacology
fascicolo: 5, volume: 117, anno: 1996,
pagine: 799 - 804
SICI:
0007-1188(1996)117:5<799:OREACT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARGININE VASOPRESSIN; MOLECULAR-CLONING; RADIOLIGAND; RAT; BINDING; SYSTEMS;
Keywords:
HUMAN VASCULAR SMOOTH MUSCLE CELL LINE; OXYTOCIN RECEPTOR; INTRACELLULAR CA2+; INOSITOL (1,4,5)-TRISPHOSPHATE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
H. Yazawa et al., "OXYTOCIN RECEPTORS EXPRESSED AND COUPLED TO CA2-MUSCLE CELL-LINE( SIGNALING IN A HUMAN VASCULAR SMOOTH)", British Journal of Pharmacology, 117(5), 1996, pp. 799-804

Abstract

1 In a human vascular smooth muscle cell line (HVSMC), binding experiments with [H-3]-arginine(8)-vasopressin (AVP) have shown the existence of a homogeneous population of binding sites with affinity (K-d value) of 0.65 nM and a maximum number of binding sites (B-max) of 122 fmol mg(-1) protein. 2 Nonlabelled compounds compete for [H-3]-AVP binding in the HVSMC membrane with an order of potency of oxytocin > lyspressin greater than or equal to AVP > Thr(4), Gly(7)-oxytocin > rcapto-beta-beta-cyclopentamethylenepropionyl-O-Me Tyr(2), Arg(8)) vasopressin > desmopressin > OPC21268 > OPC31260. This order was markedly different from that observed in rat vascular smooth muscle cells (A10), a well-established V-1A receptor system. 3 In HVSMC both oxytocin and AVP increased inositol 1,4,5-trisphosphate (IP3) production and [Ca2+](i) response, but the efficacy of the responses was greater for oxytocin than AVP. 4 Reverse transcription-polymerase chain reaction (RT-PCR) assay detected only oxytocin receptor but not V-1A Or V-2 receptors in HVSMC, whereas only V-1A receptors were found in A10 cells. 5 In conclusion, in HVSMC only oxytocin receptors are expressed among the vasopressin receptor family, and they coupled to phosphatidyl inositol (PI) turnover/Ca2+ signalling. This unexpected observation should provide new insight into the functional role of the oxytocin receptor in a human vascular smooth muscle cell line.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 10:41:47