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Titolo:
MOLECULAR CHARACTERIZATION OF KATA FROM CAMPYLOBACTER-JEJUNI AND GENERATION OF A CATALASE-DEFICIENT MUTANT OF CAMPYLOBACTER-COLI BY INTERSPECIFIC ALLELIC EXCHANGE
Autore:
GRANT KA; PARK SF;
Indirizzi:
INST FOOD RES,READING LAB,EARLEY GATE,WHITEKNIGHTS RD READING RG6 2EFBERKS ENGLAND INST FOOD RES,READING LAB READING RG6 2EF BERKS ENGLAND
Titolo Testata:
Microbiology
, volume: 141, anno: 1995,
parte:, 6
pagine: 1369 - 1376
SICI:
1350-0872(1995)141:<1369:MCOKFC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; HYDROGEN-PEROXIDE; ACID-SEQUENCE; TRANSFORMATION; CLONING; HPII; DNA;
Keywords:
CAMPYLOBACTER JEJUNI; CAMPYLOBACTER COLI; CATALASE; KATA; GENE REPLACEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
K.A. Grant e S.F. Park, "MOLECULAR CHARACTERIZATION OF KATA FROM CAMPYLOBACTER-JEJUNI AND GENERATION OF A CATALASE-DEFICIENT MUTANT OF CAMPYLOBACTER-COLI BY INTERSPECIFIC ALLELIC EXCHANGE", Microbiology, 141, 1995, pp. 1369-1376

Abstract

A gene encoding catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase; EC 1.11.1.6) from Campylobacter jejuni was cloned by functional complementation of a catalase-deficient mutant of Escherichia coli. The catalase structural gene, designated katA, was assigned by subcloning and its nucleotide sequence determined. The deduced protein product of 508 amino acids, which had a calculated molecular mass of 58346 Da. was found to be structurally and enzymically similar to hydrogen-peroxidases from other bacterial species. The region of DNA containing the structural catalase gene was disrupted by insertion of a tetracycline-resistance marker and the modified sequence then introduced into astrain of Campylobacter coli via natural transformation. Genetic and enzymic analyses of a tetracycline-resistant C. coli transformant confirmed that catalase-deficient mutants had arisen via interspecific allelic exchange. Compared to the isogenic parental strain the mutant wasmore sensitive to killing by H2O2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 08:58:46