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Titolo:
SERUM HIGH-DENSITY-LIPOPROTEINS IN THE CRAB CANCER ANTENNARIUS .4. ELECTROPHORETIC AND IMMUNOLOGICAL ANALYSES OF APOLIPOPROTEINS AND A QUESTION OF FEMALE-SPECIFIC LIPOPROTEINS
Autore:
SPAZIANI E; WANG WL; NOVY LA;
Indirizzi:
UNIV IOWA,DEPT BIOL SCI IOWA CITY IA 52242
Titolo Testata:
Comparative biochemistry and physiology. B. Comparative biochemistry
fascicolo: 2, volume: 111, anno: 1995,
pagine: 265 - 276
SICI:
0305-0491(1995)111:2<265:SHITCC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOMARUS-AMERICANUS; LIPOVITELLIN; CRUSTACEAN; HEMOLYMPH; PROTEINS; VITELLOGENESIS; LOBSTER; OVARY; IMMUNOREACTIVITY; CONFORMATION;
Keywords:
APOLIPOPROTEINS; BRACHYURA; CALLINECTES SAPIDUS; CANCER ANTENNARIUS; CRUSTACEA; FEMALE-SPECIFIC PROTEIN; LIPOPROTEINS; VITELLOGENIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
E. Spaziani et al., "SERUM HIGH-DENSITY-LIPOPROTEINS IN THE CRAB CANCER ANTENNARIUS .4. ELECTROPHORETIC AND IMMUNOLOGICAL ANALYSES OF APOLIPOPROTEINS AND A QUESTION OF FEMALE-SPECIFIC LIPOPROTEINS", Comparative biochemistry and physiology. B. Comparative biochemistry, 111(2), 1995, pp. 265-276

Abstract

Apolipoproteins (APO) were analyzed for molecular mass and specificity of occurrence in hemolymph and eggs. Hemolymph donors were males andboth ovigerous and non-ovigerous females. Total lipoprotein (LP; exclusively high-density LP) was fractionated into subclasses by density-gradient centrifugation; selected subclasses were delipidated and the APO subjected to electrophoresis under denaturing conditions, Antisera were raised against the isolated APO of both males and females and used for tests of cross-reactivity in Western blots. Principally three protein units were identified (molecular masses: 185, 100 and 84 kDa). Each occurred in the APO of each HDL subclass, in the hemolymphs of males and all females and in eggs. The antisera cross-reacted between allgroups. Similar findings were obtained in a less complete study of APO in the blue crab, Callinectes sapidus. Thus, in analyses of isolatedAPO from two crab species, no evidence could be found for occurrence of a ''female-specific protein'' (FSP). FSP has been defined as a protein (vitellogenin, more accurately an LP) that is generated only by females active in production of egg yolk LP (vitellin); it is presumed to specialize in transporting yolk lipids. The crustacean literature issurveyed to compare data on APO molecular mass and critically reviewed with regard to FSP.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:44:11