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Titolo:
CRYSTALLINE SURFACE PROTEIN OF PEPTOSTREPTOCOCCUS-ANAEROBIUS
Autore:
KOTIRANTA A; HAAPASALO M; LOUNATMAA K; KARI K;
Indirizzi:
HELSINKI UNIV,DEPT CARDIOL,PB 41 SF-00014 HELSINKI FINLAND HELSINKI UNIV,DEPT CARDIOL SF-00014 HELSINKI FINLAND HELSINKI UNIV,DEPT ELECTRON MICROSCOPY SF-00014 HELSINKI FINLAND HELSINKI UNIV,INST DENT,RES LAB SF-00014 HELSINKI FINLAND
Titolo Testata:
Microbiology
, volume: 141, anno: 1995,
parte:, 5
pagine: 1065 - 1073
SICI:
1350-0872(1995)141:<1065:CSPOP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENUS PEPTOSTREPTOCOCCUS; EUBACTERIUM-YURII; SP-NOV; MAGNUS; BACTERIA; MICROS; COCCI; ASACCHAROLYTICUS; HYDROPHOBICITY; PERIODONTITIS;
Keywords:
PEPTOSTREPTOCOCCUS; S-LAYER; SURFACE PROTEIN; ULTRASTRUCTURE; ORAL BACTERIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
A. Kotiranta et al., "CRYSTALLINE SURFACE PROTEIN OF PEPTOSTREPTOCOCCUS-ANAEROBIUS", Microbiology, 141, 1995, pp. 1065-1073

Abstract

The surface ultrastructure of three anaerobic Gram-positive cocci frequently encountered in oral infections, Peptostreptococcus micros, P. magnus and P. anaerobius, was studied. The type strains of P. micros (DSM 20468) and P. anaerobius (ATCC 27337), several clinical isolates of both species and the type strain of P. magnus (DSM 20470) were included. Thin-sectioned cells studied by electron microscopy revealed a homogeneous layer outside the peptidoglycan layer in P. anaerobius. In P. micros and P. magnus a more amorphous layer was present. No periodicstructures were seen in negatively stained whole cells of these threespecies. However, in freeze-etched cells of P. anaerobius a crystalline surface protein layer (S-layer) was detected. No periodicity was seen in any of the P. micros strains or the P. magnus type strain by themethods used, but a periodic pattern was observed in negatively stained specimens of cell wall fragments of sonicated P. anaerobius cells. No capsular material was visible outside the S-layer in P. anaerobius. The cells of the Peptostreptococcus spp. were extracted for 30 min with detergents and urea. One per cent SDS and 6 M urea both extracted amajor 78 kDa protein from all strains of P. anaerobius. Extraction ofP. micros and P. magnus cells did not reveal any major protein bands comparable to that of P. anaerobius. Surface biotinylation of cells followed by Western blotting and detection by alkaline-phosphatase-conjugated extravidin showed strong staining of the 78 kDa band in P. anaerobius, further indicating that this molecule is located on the surfaceof the cell and is the S-protein. Another protein, of 127 kDa, was also detected by surface biotinylation in all P. anaerobius strains. No differences were detected in colony morphology or cell surface structures between the four strains. Isoelectric focusing of the proteins of P. anaerobius revealed that the S-proteins of individual strains have different pI values, ranging from 5.3 to 6.9.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 15:57:58