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Titolo:
NA-23 NMR-SPECTROSCOPY OF FREE NA-COLI( IN THE HALOTOLERANT BACTERIUMBREVIBACTERIUM SP AND ESCHERICHIA)
Autore:
NAGATA S; ADACHI K; SHIRAI K; SANO H;
Indirizzi:
KOBE UNIV MERCHANTILE MARINE,MARINE CARGO TRANSPORTAT RES INST,HIGASHINADA KU KOBE HYOGO 658 JAPAN MARINE BIOTECHNOL INST CO LTD SHIMIZU SHIZUOKA 424 JAPAN IDEMITSU KOSAN CO LTD SODEGAURA CHIBA 29902 JAPAN
Titolo Testata:
Microbiology
, volume: 141, anno: 1995,
parte:, 3
pagine: 729 - 736
SICI:
1350-0872(1995)141:<729:NNOFNI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; COMPATIBLE SOLUTES; METHANOGENIC ARCHAEBACTERIA; HALOPHILIC EUBACTERIA; VIBRIO-ALGINOLYTICUS; SALT CONCENTRATIONS; TRANSPORT; SODIUM; OSMOREGULATION; ACCUMULATION;
Keywords:
NA-23 NMR; SODIUM IONS; HALOTOLERANCE; BREVIBACTERIUM SP; ESCHERICHIA COLI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
S. Nagata et al., "NA-23 NMR-SPECTROSCOPY OF FREE NA-COLI( IN THE HALOTOLERANT BACTERIUMBREVIBACTERIUM SP AND ESCHERICHIA)", Microbiology, 141, 1995, pp. 729-736

Abstract

Na-23 NMR spectroscopy was used to determine free Na+ concentrations in a halotolerant bacterium, Brevibacterium sp., and Escherichia coli. The internal Na+ concentration of both strains depended little on thegrowth phases and was unchanged after 5 d storage at 2 degrees C. In Brevibacterium, sp. the level of intracellular sodium increased gradually at higher extracellular NaCl concentrations in both the presence and absence of yeast extract in the growth medium. E. coli cells accumulated a higher concentration of free Na+ than those of Brevibacterium sp. The change of Na+ concentration in both strains was inverse to that of growth rate. When appropriate amounts of osmoprotectants (proline. glycine betaine, or gamma-aminobutyrate) were added with the NaCl, internal free Na-+ levels in Brevibacterium sp. were lowered, but thoseof E. coli were unchanged. While addition of KCl to medium containingNaCl increased the intracellular level of free Na+, the total sodium concentration in the cells remained unchanged, indicating that sodium that had been bound or attached was made free in the cytosol. In Brevibacterium sp. grown in the presence of 0.5 M NaCl, free and bound sodium concentrations in the cytosol were estimated to be 0.14 and 0.23 pmol (mg protein)(-1), respectively. As a result, visibility by Na-23 NMR was 38%.

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Documento generato il 29/11/20 alle ore 03:23:06