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Titolo:
STRUCTURAL REQUIREMENTS FOR NEUROPEPTIDE-Y IN MAST-CELL AND G-PROTEINACTIVATION
Autore:
MOUSLI M; TRIFILIEFF A; PELTON JT; GIES JP; LANDRY Y;
Indirizzi:
UNIV CALIF SAN DIEGO,DIV CELLULAR & MOLEC MED,9500 GILMAN DR LA JOLLACA 92093 UNIV LOUIS PASTEUR STRASBOURG 1,INSERM,U425 ILLKIRCH GRAFFENS FRANCE MARION MERRELL DOW RES INST STRASBOURG FRANCE
Titolo Testata:
European journal of pharmacology. Molecular pharmacology section
fascicolo: 1, volume: 289, anno: 1995,
pagine: 125 - 133
SICI:
0922-4106(1995)289:1<125:SRFNIM>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERTUSSIS TOXIN; ADENYLATE-CYCLASE; HISTAMINE-RELEASE; SUBSTANCE-P; PEPTIDE-YY; BINDING; MASTOPARAN; MECHANISMS; FRAGMENTS; RECEPTORS;
Keywords:
MAST CELL; NEUROPEPTIDE Y; CIRCULAR DICHROISM SPECTRUM; HETEROTRIMERIC G PROTEIN; BENZALKONIUM CHLORIDE; PERTUSSIS TOXIN; (RAT); (HUMAN);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
M. Mousli et al., "STRUCTURAL REQUIREMENTS FOR NEUROPEPTIDE-Y IN MAST-CELL AND G-PROTEINACTIVATION", European journal of pharmacology. Molecular pharmacology section, 289(1), 1995, pp. 125-133

Abstract

Incubation of neuropeptide Y or its C-terminal fragments with rat peritoneal mast cells resulted in a dose-dependent histamine release. Fragment 18-36 of neuropeptide Y was the most biologically active peptide. EC(25) value on rat mast cells was 7.2 +/- 2.2 nM. Neuropeptide Y was also able to induce a flare response after intradermal injection in humans. The histamine releasing effects of neuropeptide Y related peptides were greatly inhibited by pretreatment of rat mast cells with pertussis toxin or benzalkonium chloride. Neuropeptide Y and C-terminal related peptides also stimulated the GTPase activity of purified heterotrimeric G proteins in a dose-dependent manner from 1 to 50 mu M. Binding studies with [I-125]neuropeptide Y were unable to provide evidencefor the presence of specific binding sites on the surface of mast cells. The alpha helical conformation of neuropeptide Y fragments was studied by measuring the circular dichroism spectra. Neuropeptide Y-(18-36) was the smallest fragment having a strong helical conformation. Ourresults demonstrate that neuropeptide Y activates mast cells through a non-specific process leading to G protein activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/10/20 alle ore 00:12:01