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Titolo:
THE ROLE OF CARBON-MONOXIDE IN LUCIGENIN-DEPENDENT CHEMILUMINESCENCE OF RAT ALVEOLAR MACROPHAGES
Autore:
FUKUSHIMA T; OKINAGA S; SEKIZAWA K; OHRUI T; YAMAYA M; SASAKI H;
Indirizzi:
TOHOKU UNIV,SCH MED,DEPT GERIATR MED,AOBA KU,SEIRYO MACHI 1-1 SENDAI MIYAGI 980 JAPAN
Titolo Testata:
European journal of pharmacology. Molecular pharmacology section
fascicolo: 1, volume: 289, anno: 1995,
pagine: 103 - 107
SICI:
0922-4106(1995)289:1<103:TROCIL>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; HEME OXYGENASE; CYCLIC-GMP; L-ARGININE; GUANYLATE-CYCLASE; CELL; GRANULOCYTES; INHIBITION; ACTIVATION; EXPRESSION;
Keywords:
ALVEOLAR MACROPHAGE; CARBON MONOXIDE (CO); NITRIC OXIDE (NO); HEME OXYGENASE; CYCLIC GMP; SUPEROXIDE ANION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
T. Fukushima et al., "THE ROLE OF CARBON-MONOXIDE IN LUCIGENIN-DEPENDENT CHEMILUMINESCENCE OF RAT ALVEOLAR MACROPHAGES", European journal of pharmacology. Molecular pharmacology section, 289(1), 1995, pp. 103-107

Abstract

We have investigated the role of carbon monoxide (CO) in lucigenin-dependent chemiluminescence of alveolar macrophages from rat lungs. CO (10 nM to 1 mu M) decreased chemiluminescence of alveolar macrophages in a concentration-dependent fashion. At a concentration of 1 mu M, CO significantly increased intracellular cyclic GMP levels from a controlvalue of 175 +/- 25 fmol/2 x 10(6) cells to 431 +/- 49 fmol/2 x 10(6)cells. Pretreatment of alveolar macrophages with N-G-monomethyl-L-arginine (100 mu M) failed to inhibit CO (1 mu M)-induced decreases in chemiluminescence of alveolar macrophages (3.7 +/- 0.7 cpm X 10(3) in the presence of N-G-monomethyl-L-arginine and 3.4 +/- 0.6 cpm X 10(3) inthe absence of N-G-monomethyl-L-arginine) and CO (1 mu M)-induced increases in intracellular cyclic GMP levels (452 +/- 65 fmol/2 X 10(6) cells in the presence of N-G-monomethyl-L-arginine and 419 +/- 58 fmol/2 x 10(6) cells in the absence of N-G-monomethyl-L-arginine). Decreases in chemiluminescence of alveolar macrophages induced by CO (1 mu M) were concentration-dependently inhibited by methylene blue (from 0.1 mu M to 10 mu M). Dibutyryl cyclic GMP (db cyclic GMP) (1 mM) also reduced chemiluminescence of alveolar macrophages (1.5 +/- 0.3 cpm x 10(3)in the presence of db cyclic GMP and 3.6 +/- 0.6 cpm X 10(3) in the absence of db cyclic GMP). In contrast to CO and db cyclic GMP, zinc protoporphyrin-9 (10 nM to 1 mu M), an inhibitor of heme oxygenase potentiated chemiluminescence of alveolar macrophages in a concentration-dependent fashion. Northern blot analysis revealed that alveolar macrophages expressed mRNA of heme oxygenase-1. These results suggest that anendogenous CO-like factor inhibits chemiluminescence of alveolar macrophages via activating guanylate cyclase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 19:32:08