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Titolo:
GROWTH OF ARTHROBACTER-GLOBIFORMIS IN SOIL OBSERVED BY FLUORESCENT-ANTIBODY AND ELISA TECHNIQUES
Autore:
MANSOOR EY; GRAY TRG;
Indirizzi:
UNIV ESSEX,DEPT BIOL,WIVENHOE PK COLCHESTER CO4 3SQ ESSEX ENGLAND UNIV ESSEX,DEPT BIOL COLCHESTER CO4 3SQ ESSEX ENGLAND
Titolo Testata:
Microbiology
, volume: 141, anno: 1995,
parte:, 2
pagine: 505 - 511
SICI:
1350-0872(1995)141:<505:GOAISO>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED IMMUNOSORBENT-ASSAY; DISTINCT BACTERIA FLAVOBACTERIUM; RHIZOSPHERE SOIL; IDENTIFICATION; MICROORGANISMS; INOCULANTS; SURVIVAL; STRAINS;
Keywords:
SOIL MICROBIOLOGY; ARTHROBACTER GLOBIFORMIS; CELL ENUMERATION; FLUORESCENT ANTIBODIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
E.Y. Mansoor e T.R.G. Gray, "GROWTH OF ARTHROBACTER-GLOBIFORMIS IN SOIL OBSERVED BY FLUORESCENT-ANTIBODY AND ELISA TECHNIQUES", Microbiology, 141, 1995, pp. 505-511

Abstract

The growth and survival of Arthrobacter globiformis (NCIMB 10683) in a wheatfield and a sand-dune soil were investigated by introducing rod-shaped cells on the surface of microscope slides into the soil. Slides were recovered and stained with fluorescent antibodies. In the wheatfield soil, new microcolonies of considerable size were observed on oraround both soil particles and fungal hyphae throughout the incubation period of 48 d. In both sterile and non-sterile wheatfield soil, themajority of the rods reverted to cocci after 2 weeks incubation. In the sand-dune soil, cells did not grow and only a few were found after 48 d. Growth of A. globiformis in the wheatfield soil was also investigated using a direct soil inoculation technique. Soil samples were recovered after different incubation periods and cells extracted using a centrifugation method. The number of cells in extracts was estimated using ELISA and direct count techniques. In the latter technique, extracted cells were double-stained with fluorescent antibodies and ethidium bromide. Growth took place in both sterile and non-sterile wheatfield soil. The introduced rods reverted to cocci after a few days, as on the slides. However, cells introduced directly into soil grew faster than those introduced on slides.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 09:39:39