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Titolo:
DOPAMINE D-2 AND D-3 RECEPTORS IN THE RAT STRIATUM AND NUCLEUS-ACCUMBENS - USE OF 7-OH-DPAT AND [I-125] IODOSULPRIDE
Autore:
BOOZE RM; WALLACE DR;
Indirizzi:
UNIV KENTUCKY,COLL MED,DEPT PHARMACOL,UKMC,MS-305 LEXINGTON KY 40536
Titolo Testata:
Synapse
fascicolo: 1, volume: 19, anno: 1995,
pagine: 1 - 13
SICI:
0887-4476(1995)19:1<1:DDADRI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
H-3 QUINPIROLE BINDING; AUTORADIOGRAPHIC LOCALIZATION; BRAIN DOPAMINE; I-125 IODOSULPRIDE; INVITRO BINDING; MESSENGER-RNA; D1; INHIBITION; EXPRESSION; SYSTEMS;
Keywords:
DOPAMINERGIC; VENTRAL STRIATUM; CAUDATE-PUTAMEN; NUCLEUS ACCUMBENS; AUTORADIOGRAPHY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
72
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Booze e D.R. Wallace, "DOPAMINE D-2 AND D-3 RECEPTORS IN THE RAT STRIATUM AND NUCLEUS-ACCUMBENS - USE OF 7-OH-DPAT AND [I-125] IODOSULPRIDE", Synapse, 19(1), 1995, pp. 1-13

Abstract

A novel dopamine receptor mRNA transcript has been identified and classified as the D-3 receptor subtype. We have examined the binding of the D-2/D-3-selective compound [I-125]-Iodosulpride using unlabeled D-3-selective 7-OH-DPAT to determine the distribution of D-2 and D-3 dopamine receptor subtypes in the rat basal forebrain. Use of[I-125]-labeled ligands has significant advantages over [H-3]-labeled compounds forautoradiographic studies, especially for evaluating small brain areascontaining low receptor densities. [I-125]-Iodosulpride identified two sites with high affinity (< 1 nM) in the presence of (-)sulpiride (1mu M; D-2+3) or 7-OH-DPAT (10 nM; D-3), with a greater density of D-2receptors (twofold) compared to D-3 receptors in the striatum. The density of D-2 and D-3 receptor subtypes displayed a 1:1 ratio in the nucleus accumbens. [I-125]- Iodosulpride with 7-OH-DPAT displayed D-2 sites, predominately in the striatum. Digital subtraction autoradiography showed the highest levels of D-3 binding in the islands of Calleja as well as in the core and shell regions of the nucleus accumbens. In sum, the advantages in using [I-125]-Iodosulpride to label the dopaminereceptor subtypes are high specific activity, affinity, and lack of quenching in autoradiographic analyses. Moreover, masking D-3 receptorswith 7-OH-DPAT permitted indirect determination of D-3 receptor density and localization using the [I-125]-labeled ligand, without the potential confound of 7-OH-DPAT binding to sigma receptors. The colocalization of the D-2 dopamine receptors with D-3 receptors suggests that unique interactions may exist between the receptor subtypes in the rat basal forebrain region. (C) 1995 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 07:21:27