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Titolo:
IN-VITRO INDUCTION OF CYTOTOXIC T-LYMPHOCYTES AGAINST HTLV-I-INFECTEDT-CELLS FROM ADULT T-CELL LEUKEMIA PATIENTS, ASYMPTOMATIC HTLV-I CARRIERS AND SERONEGATIVE HEALTHY DONORS
Autore:
KATAHIRA Y; YASHIKI S; FUJIYOSHI T; NOMURA K; TARA M; MORI M; SETOYAMA M; KANZAKI T; SHIDA H; SONODA S;
Indirizzi:
KAGOSHIMA UNIV,FAC MED,DEPT DERMATOL,SAKURAGOAKA 8-35-1 KAGOSHIMA 890JAPAN KAGOSHIMA UNIV,FAC MED,DEPT DERMATOL KAGOSHIMA 890 JAPAN HOKUSATSU HOSP OKUCHI KANAGAWA 89525 JAPAN KAGOSHIMA CITY HOSP KAGOSHIMA 890 JAPAN MED COLL OITA,DEPT INTERNAL MED 3 OITA 87955 JAPAN KYOTO UNIV,INST VIRUS RES,SAKYO KU KYOTO 606 JAPAN
Titolo Testata:
Japanese journal of cancer research
fascicolo: 1, volume: 86, anno: 1995,
pagine: 21 - 27
SICI:
0910-5050(1995)86:1<21:IIOCTA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIRUS TYPE-I; MONOCLONAL-ANTIBODY; LYMPHOMA; ANTIGENS; LINE; LEUKOCYTES; INVITRO; PROTEIN; PX;
Keywords:
ANTI-HTLV-I CTL; IN VITRO INDUCTION OF CTL; ATL; ASYMPTOMATIC HTLV-I CARRIER; HTLV-I SERONEGATIVE HEALTHY DONOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
Y. Katahira et al., "IN-VITRO INDUCTION OF CYTOTOXIC T-LYMPHOCYTES AGAINST HTLV-I-INFECTEDT-CELLS FROM ADULT T-CELL LEUKEMIA PATIENTS, ASYMPTOMATIC HTLV-I CARRIERS AND SERONEGATIVE HEALTHY DONORS", Japanese journal of cancer research, 86(1), 1995, pp. 21-27

Abstract

We investigated an in vitro method to produce cytotoxic T lymphocytes(CTLs) against HTLV-I-infected T-cells using peripheral blood mononuclear cells (PBMC) of adult T-cell leukemia (ATL) patients, asymptomatic HTLV-I carriers (AC) and seronegative healthy donors. The PBMC were restimulated repeatedly for 4 weeks with HLA-matched HTLV-I-infected T-cells which had been pretreated at 56 degrees C for 30 min to inactivate infectious HTLV-I. The culture medium included 10-100 units/ml of recombinant lymphokines (rIL-1, rIL-2, rIL-4, rIL-6 and rIL-7) and 10%fetal calf serum in RPMI-1640 medium, The cytotoxic activity was measured against HLA-matched HTLV-I-infected T-cell lines after CD4(+) or CD8(+) cells were positively panned from the cultured PBMC. The PBMC of ATL, AC and healthy donors were able to produce either CD4(+) or CD8(+) CTLs against HTLVI-related antigens (env, gag, p21(chi), p27(rex) and p40(tax)) as well as the antigen(s) of as-yet unknown specificity expressed on HTLV-I-infected T-cells. All the CTLs recognized the specific antigens in the context of either class I or class II HLA types. These results indicated that ATL patients, AC and healthy donors were immunocompetent to generate CTLs against HTLV-I-infected T-cells and probably against HTLV-I-transformed T-cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 12:27:38