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Titolo:
THE EFFECTS OF CORTICOSTEROIDS ON LYMPHOCYTE RECIRCULATION IN HUMANS - ANALYSIS OF THE MECHANISM OF IMPAIRED LYMPHOCYTE MIGRATION TO LYMPH-NODE FOLLOWING METHYLPREDNISOLONE ADMINISTRATION
Autore:
SACKSTEIN R; BORENSTEIN M;
Indirizzi:
UNIV S FLORIDA,COLL MED,H LEE MOFFITT CANC CTR,DEPT INTERNAL MED,DIV BONE MARROW TRANSPLANTAT TAMPA FL 33612 UNIV S FLORIDA,COLL MED,H LEE MOFFITT CANC CTR,DEPT PATHOL,DIV BONE MARROW TRANSPLANTAT TAMPA FL 33612 UNIV S FLORIDA,COLL MED,H LEE MOFFITT CANC CTR,DEPT LAB MED,DIV BONE MARROW TRANSPLANTAT TAMPA FL 33612 UNIV MIAMI,SCH MED,MIAMI VET AFFAIRS MED CTR,DEPT MED MIAMI FL 00000
Titolo Testata:
Journal of investigative medicine
fascicolo: 1, volume: 43, anno: 1995,
pagine: 68 - 77
SICI:
1081-5589(1995)43:1<68:TEOCOL>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYMUS-DERIVED LYMPHOCYTES; HOMING RECEPTORS; BONE-MARROW; HIGH ENDOTHELIUM; IMMUNE-SYSTEM; ADHESION; HYDROCORTISONE; SUBPOPULATIONS; CELLS; PREDNISOLONE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
R. Sackstein e M. Borenstein, "THE EFFECTS OF CORTICOSTEROIDS ON LYMPHOCYTE RECIRCULATION IN HUMANS - ANALYSIS OF THE MECHANISM OF IMPAIRED LYMPHOCYTE MIGRATION TO LYMPH-NODE FOLLOWING METHYLPREDNISOLONE ADMINISTRATION", Journal of investigative medicine, 43(1), 1995, pp. 68-77

Abstract

Background: Steroid therapy profoundly inhibits lymphocyte migration into lymph nodes (LN), thereby disrupting lymphocyte recirculation. This study was undertaken in human subjects to examine two nonmutually exclusive hypotheses proposed to explain this steroid effect: (1) steroids decrease the capacity of LN high endothelial venules (HEV) to support lymphocyte adherence; and/or (2) steroids alter the expression of lymphocyte adhesion molecules specific for entry into LNs. Methods: Human LNs were obtained from cadaveric organ donors before and after methylprednisolone infusion and examined for their capacity to support lymphocyte adherence using an in vitro lymphocyte-HEV adherence assay. Additionally, six healthy volunteers underwent infusions of saline (placebo) and methylprednisolone (3 mg/kg in saline), and peripheral bloodlymphocytes were isolated before, immediately after, and at 1, 2, 4, 8, 24, and 48 hours following infusions. At each timepoint, flow cytometric analysis of lymphocyte L-selectin, CD44, and LFA-1 expression was assessed. Short-term tissue culture studies were also performed to examine the direct effects of steroids on the expression of these lymphocyte proteins. Results: LNs obtained from organ donors before and after steroid infusion displayed no differences in the capacity of HEV tosupport lymphocyte adherence. In controlled steroid infusion studies,lymphopenia was observed within 1 hour and persisted for 8 hours. Cells isolated during the period of lymphopenia did not adhere to LN HEV,and there was a marked decrease in both the percentage and mean fluorescence intensity level of L-selectin(+) lymphocytes; minor changes were observed in the percentage of CD44(+) and LFA-1(+) cells, while themean fluorescence intensity level decreased for CD44(+) cells and increased for LFA-1(+) cells. All such changes in adhesion protein levelsamong circulating cells reversed with the resolution of lymphopenia. However, in tissue culture experiments, steroids did not alter the expression of lymphocyte adhesion proteins. Conclusions: The decreased migration of lymphocytes to LN following steroid administration is not due to changes in the capacity of HEV to bind lymphocytes, but results from decreases in the level of L-selectin expressed among circulating cells. Culture studies suggest that steroids do not directly alter lymphocyte adhesion protein expression. The possibility that these agentsact indirectly by stimulating the release and/or promoting the activity of relevant biologic mediators affecting lymphocyte adhesion protein expression needs further exploration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:18:17