Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SEQUENCE-INDEPENDENT DETECTION OF GENE FAMILY HOMOLOGS - IDENTIFICATION OF A TRANSCRIPT ENCODING A MOLLUSCAN SERINE-PROTEASE HOMOLOGOUS TO THE PANCREATIC-ENZYMES OF VERTEBRATES
Autore:
GROPPE JC; MORSE DE;
Indirizzi:
UNIV CALIF SANTA BARBARA,DEPT BIOL SCI SANTA BARBARA CA 93106 UNIV CALIF SANTA BARBARA,DEPT BIOL SCI SANTA BARBARA CA 93106 UNIV CALIF SANTA BARBARA,CTR MARINE BIOTECHNOL SANTA BARBARA CA 93106
Titolo Testata:
Comparative biochemistry and physiology. B. Comparative biochemistry
fascicolo: 1, volume: 110, anno: 1995,
pagine: 75 - 82
SICI:
0305-0491(1995)110:1<75:SDOGFH>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; ACID; RIBONUCLEASE; ANGLERFISH; CLONING; CDNAS; DNA;
Keywords:
CDNAS; GENE FAMILY HOMOLOGS; TRANSCRIPT; MOLLUSCAN SERINE PROTEASE; PANCREATIC ENZYMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
J.C. Groppe e D.E. Morse, "SEQUENCE-INDEPENDENT DETECTION OF GENE FAMILY HOMOLOGS - IDENTIFICATION OF A TRANSCRIPT ENCODING A MOLLUSCAN SERINE-PROTEASE HOMOLOGOUS TO THE PANCREATIC-ENZYMES OF VERTEBRATES", Comparative biochemistry and physiology. B. Comparative biochemistry, 110(1), 1995, pp. 75-82

Abstract

Autoradiography of P-32-labeled cDNA, fractionated at high resolutionby electrophoresis through thin (0.8-1.5 mm) vertical alkaline agarose gels, provides a sequence-independent screening procedure for gene family homologs, A screen of tissues of a marine mollusc revealed a prominent intestine-specific cDNA encoding a pancreatic serine protease homolog, which was not detectable as a discrete poly(A)(+) RNA species on formaldehyde agarose gels, Discrete cDNA products are authentic, non-truncated transcripts of tissue-specific mRNA. A band-sharpening effect is imparted to cDNA products due to (a) substitution of a uniform length 5'-oligo(dT) terminus for heterogeneous 3'-poly(A) termini rind(b) the inherent superior resolution of alkaline-denatured DNA,

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 16:45:10