Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A COMPARATIVE-STUDY OF PREPARATION TECHNIQUES FOR IMPROVING THE VIABILITY OF STRIATAL GRAFTS USING VITAL STAINS, IN-VITRO CULTURES, AND IN-VIVO GRAFTS
Autore:
FRICKER RA; BARKER RA; FAWCETT JW; DUNNETT SB;
Indirizzi:
UNIV CAMBRIDGE,MRC,CAMBRIDGE CTR BRAIN REPAIR,FORVIE SITE,ROBINSON WAY CAMBRIDGE CB2 2PY ENGLAND UNIV CAMBRIDGE,DEPT EXPT PSYCHOL CAMBRIDGE CB2 2PY ENGLAND UNIV CAMBRIDGE,DEPT PHYSIOL CAMBRIDGE CB2 2PY ENGLAND UNIV CAMBRIDGE,MRC,CAMBRIDGE CTR BRAIN REPAIR CAMBRIDGE CB2 2PY ENGLAND
Titolo Testata:
Cell transplantation
fascicolo: 6, volume: 5, anno: 1996,
pagine: 599 - 611
SICI:
0963-6897(1996)5:6<599:ACOPTF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-LESIONED STRIATUM; SKILLED FORELIMB USE; HUNTINGTONS-DISEASE; NEOSTRIATAL TRANSPLANTS; NIGROSTRIATAL DOPAMINE; ROTATIONAL BEHAVIOR; CAUDATE-PUTAMEN; ANIMAL-MODEL; CNS TISSUE; RAT;
Keywords:
EMBRYONIC STRIATAL GRAFTS; VITAL DYES; IN VITRO; TRYPSIN; AGE; TRITURATION PROCESS; APOMORPHINE ROTATION; PAW REACHING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
R.A. Fricker et al., "A COMPARATIVE-STUDY OF PREPARATION TECHNIQUES FOR IMPROVING THE VIABILITY OF STRIATAL GRAFTS USING VITAL STAINS, IN-VITRO CULTURES, AND IN-VIVO GRAFTS", Cell transplantation, 5(6), 1996, pp. 599-611

Abstract

Cell suspension grafts from embryonic striatal primordia placed into the adult rat striatum survive well and are able to alleviate a numberof behavioral deficits caused by excitotoxic lesions to this structure. However, neither the anatomical connectivity between the graft and host nor the functional recovery elicited by the grafts is completely restored. One way in which the survival and function of embryonic striatal grafts may be enhanced is by the improvement of techniques for the preparation of the cell suspension prior to implantation, an issue that has been addressed only to a limited extent. We have evaluated a number of parameters during the preparation procedure, looking at the effects on cell survival over the first 24 h from preparation using vital dyes and the numbers of surviving neurons in vitro, after 4 days inculture, in addition to graft survival and function in vivo. Factors influencing cell survival include the type of trypsinization procedureand the age of donor tissues used for suspension preparation. The presence of DNase has no effect on cell viability but aids the dissociation of the tissue to form single cells. These results have important implications for the use of embryonic striatal grafts in animal models of Huntington's disease, and in any future clinical application of thisresearch. Copyright (C) 1996 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 00:38:56