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Titolo:
PROTEIN-KINASE-C PLAYS A KEY ROLE IN THE CROSS-TALK BETWEEN INTRACELLULAR SIGNALINGS VIA PROSTANOID RECEPTORS IN A MEGAKARYOBLASTIC CELL-LINE - MEG-01S
Autore:
WATANABE T; SUNAGA S; TOGO M; SATOH H; HIGASHIHARA M; HASHIMOTO Y; KUROKAWA K;
Indirizzi:
UNIV TOKYO,FAC MED,DEPT INTERNAL MED 1,BUNKYO KU,7-3-1 HONGO TOKYO 113 JAPAN
Titolo Testata:
Biochimica et biophysica acta, L. Lipids and lipid metabolism
fascicolo: 2, volume: 1304, anno: 1996,
pagine: 161 - 169
SICI:
0005-2760(1996)1304:2<161:PPAKRI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROSTAGLANDIN-E RECEPTOR; INOSITOL PHOSPHATE PRODUCTION; PLATELET ADENYLATE-CYCLASE; BETA-ADRENERGIC-RECEPTOR; S49 LYMPHOMA-CELLS; CYCLIC-AMP; PHORBOL ESTERS; THROMBOXANE-A2 RECEPTOR; PROSTACYCLIN RECEPTOR; CA2+ MOBILIZATION;
Keywords:
PROSTANOID RECEPTOR; CROSS-TALK; PROTEIN KINASE C; CALCIUM ION, INTRACELLULAR; CAMP; MEGAKARYOCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
T. Watanabe et al., "PROTEIN-KINASE-C PLAYS A KEY ROLE IN THE CROSS-TALK BETWEEN INTRACELLULAR SIGNALINGS VIA PROSTANOID RECEPTORS IN A MEGAKARYOBLASTIC CELL-LINE - MEG-01S", Biochimica et biophysica acta, L. Lipids and lipid metabolism, 1304(2), 1996, pp. 161-169

Abstract

In a previous study, we characterized prostanoid and thrombin receptors expressed on a megakaryoblastic cell line, MEG-01s (Blood 78, 2328-2336, 1991). In this study, we examines the mechanism of cross-talk between intracellular Ca2+ ([Ca2+](i)) and cAMP signalings through prostanoid and thrombin receptors. Addition of a thromboxane (TX)A(2) mimetic (U46619 or STA(2)) or thrombin stimulated the formation of inositolphosphates and dose-dependently augmented a prostaglandin (PG)I-2 mimetic (iloprost)- or forskolin-induced cAMP formation. 12-O-tetradecanoylphorbol-13-acetate (TPA) and ionomycin, to lesser extent, also augmented iloprost-induced cAMP formation. The enhancing effect of U46619 or TPA on cAMP formation was inhibited by prolonged pretreatment of thecells with TPA (2.5 mu M, 24 h), but not with calmodulin-antagonists;W-7, W-5, or KN-62. The elevation of [Ca2+](i) induced by thrombin, STA(2) or PGE(2) was significantly suppressed by pretreatment of the cells with TPA (100 nM) as well as cAMP mimetics such as dibutyryl cAMP (5 mM), forskolin (5 mu M) and iloprost (1 mu M). These results suggest the key role of PKC on the cross-talk between [Ca2+](i) and cAMP signalings through prostanoid and thrombin receptors; PKC, which is activated with TXA(2), or thrombin, concomitantly suppress further [Ca2+](i) elevation and enhances the PGI(2) receptor-mediated cAMP formation, which, in turn, suppress [Ca2+](i) elevation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:33:55