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Titolo:
QUANTITATIVE-EVALUATION OF BLOOD-BRAIN-BARRIER PERMEABILITY FOLLOWINGMIDDLE CEREBRAL-ARTERY OCCLUSION IN RATS
Autore:
BELAYEV L; BUSTO R; ZHAO WZ; GINSBERG MD;
Indirizzi:
UNIV MIAMI,SCH MED,DEPT NEUROL,CEREBRAL VASC DIS RES CTR,POB 016960 MIAMI FL 33101 UNIV MIAMI,SCH MED,DEPT NEUROL,CEREBRAL VASC DIS RES CTR MIAMI FL 33101
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 739, anno: 1996,
pagine: 88 - 96
SICI:
0006-8993(1996)739:1-2<88:QOBPF>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
EVANS BLUE FLUORESCENCE; FOCAL ISCHEMIA; VASCULAR-PERMEABILITY; REPERFUSION INJURY; PROTEIN TRACERS; EDEMA FORMATION; ANTAGONIST; INFARCTION; ALBUMIN; HU-211;
Keywords:
FOCAL CEREBRAL ISCHEMIA; BLOOD-BRAIN BARRIER; EVANS BLUE; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
L. Belayev et al., "QUANTITATIVE-EVALUATION OF BLOOD-BRAIN-BARRIER PERMEABILITY FOLLOWINGMIDDLE CEREBRAL-ARTERY OCCLUSION IN RATS", Brain research, 739(1-2), 1996, pp. 88-96

Abstract

A sensitive quantitative fluorescence method was used to explore the time course and regional pattern of blood-brain barrier (BBB) opening after transient middle cerebral artery occlusion (MCAo). Male Sprague-Dawley rats were anesthetized with halothane and subjected to 2 h of temporary MCAo by retrograde insertion of an intraluminal nylon suture,coated with poly-L-lysine, through the external carotid artery into the internal carotid artery and MCA. Damage to the BBB was judged by extravasation of Evans Blue (EB) dye, which was administered either 2, 3.24 or 48 h after onset of MCAo. Fluorometric quantitation of EB was performed 1 or 2 h later in six brain regions. Cerebral infarction volumes were quantitated from histopathological material at 72 h. EB extravasation first became grossly visible in the ipsilateral caudoputamen and neocortex following 3 h of MCAo, was grossly unapparent at 24-26 h, and was maximal at 48-50 h. Fluorescence quantitation confirmed thatBBB opening was absent at 2-3 h but present at all later times. In the hemisphere ipsilateral to MCAo, a 179% mean increase in extravasation of EB (compared to sham rats) was measured at 4 h, 407% at 5 h, 311%at 26 h adn 264% at 50 h. (in each case, P < 0.05 vs. sham). The volume of infarcted tissue at 72 h in this model was 163+/-7.7 mm(3). Our results indicate that an initial, acute disruption of the BBB occurs between 3 an 5 h following MCAo, and that a later, more widespread increase in regional BBB permeability is present at 48 h. Regional measurement of Evans Blue extravasation offers a precise means of quantitating BBB disruption in focal cerebral ischemia; this method will be of considerable utility is assessing the BBB-protective properties of pharmacological agents.

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Documento generato il 02/10/20 alle ore 01:38:05