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Titolo:
ACTIVE AND INACTIVE GENES LOCALIZE PREFERENTIALLY IN THE PERIPHERY OFCHROMOSOME TERRITORIES
Autore:
KURZ A; LAMPEL S; NICKOLENKO JE; BRADL J; BENNER A; ZIRBEL RM; CREMER T; LICHTER P;
Indirizzi:
DEUTSCH KREBSFORSCHUNGSZENTRUM,ABT ORGAN KOMPLEXER GENOME,NEUENHEIMERFELD 280 D-69120 HEIDELBERG GERMANY DEUTSCH KREBSFORSCHUNGSZENTRUM,ABT ORGAN KOMPLEXER GENOME D-69120 HEIDELBERG GERMANY UNIV HEIDELBERG,INST HUMAN GENET & ANTHROPOL D-6900 HEIDELBERG GERMANY UNIV HEIDELBERG,INST ANGEW PHYS HEIDELBERG GERMANY DEUTSCH KREBSFORSCHUNGSZENTRUM,BIOSTAT ABT HEIDELBERG GERMANY
Titolo Testata:
The Journal of cell biology
fascicolo: 5, volume: 135, anno: 1996,
pagine: 1195 - 1205
SICI:
0021-9525(1996)135:5<1195:AAIGLP>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE INSITU HYBRIDIZATION; POLYMERASE CHAIN-REACTION; HYBRID CELL-LINES; INTERPHASE NUCLEI; DNA-SEQUENCES; GENOMIC DISTANCE; DYSTROPHIN GENE; X-CHROMOSOME; RNA; ORGANIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
79
Recensione:
Indirizzi per estratti:
Citazione:
A. Kurz et al., "ACTIVE AND INACTIVE GENES LOCALIZE PREFERENTIALLY IN THE PERIPHERY OFCHROMOSOME TERRITORIES", The Journal of cell biology, 135(5), 1996, pp. 1195-1205

Abstract

The intranuclear position of a set of genes was analyzed with respectto the territories occupied by the whole chromosomes in which these genes are localized. Genes and their respective chromosome territories were simultaneously visualized in three-dimensionally preserved nucleiapplying dual color fluorescence in situ hybridization. Three coding (DMD, MYH7, and HBB) and two noncoding sequences (D1Z2 and an anonymous sequence) were analyzed in four different cell types, including cells where DMD and MYH7 are actively transcribed. Spatial analysis by confocal laser scanning microscopy revealed that the genes are preferentially located in the periphery of chromosome territories. This positioning was independent from the activity of the genes. In contrast, the non-expressed anonymous fragment was found randomly distributed or localized preferentially in the interior of the corresponding chromosome territory. Furthermore, the distribution of the analyzed genes within the territorial peripheries was found to be highly characteristic for each gene, and, again, independent from its expression. The impact of these findings with regard to the three-dimensional arrangement of the linear DNA string within chromosome territories, as well as with respect to a putative nuclear subcompartment confining gene expression, arediscussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 00:37:59