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Titolo:
GENE CLONING AND EXPRESSION OF NEW TREHALOSE-PRODUCING ENZYMES FROM THE HYPERTHERMOPHILIC ARCHAEUM SULFOLOBUS-SOLFATARICUS KM1

Autore:

KOBAYASHI K; KATO M; MIURA Y; KETTOKU M; KOMEDA T; IWAMATSU A;

Indirizzi:: KIRIN BREWERY CO LTD,APPL BIORES CTR,3 MIYAHARA CHO TAKASAKI GUMMA 37012 JAPAN KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,KANAZAWA KU YOKOHAMA KANAGAWA 236 JAPAN
Titolo Testata:: Bioscience, biotechnology, and biochemistry
fascicolo: 11, volume: 60, anno: 1996,
pagine: 1882 - 1885
SICI:: 0916-8451(1996)60:11<1882:GCAEON>2.0.ZU;2-W
Fonte:: ISI
Lingua:: ENG
Soggetto:: ARCHAEBACTERIUM PYROCOCCUS-FURIOSUS; THERMOSTABLE ALPHA-AMYLASE;
Keywords:: TREHALOSE; SULFOLOBUS; GLYCOSYLTRANSFERASE; GLYCOSYLTREHALOSE; ALPHA-AMYLASE FAMILY;
Tipo documento:: Article
Natura:: Periodico
Settore Disciplinare:: Science Citation Index Expanded; Science Citation Index Expanded; Science Citation Index Expanded; Science Citation Index Expanded
Citazioni:: 18
Recensione:
Indirizzi per estratti:



Citazione:: K. Kobayashi et al., "GENE CLONING AND EXPRESSION OF NEW TREHALOSE-PRODUCING ENZYMES FROM THE HYPERTHERMOPHILIC ARCHAEUM SULFOLOBUS-SOLFATARICUS KM1", Bioscience, biotechnology, and biochemistry, 60(11), 1996, pp. 1882-1885

Abstract

The genes encoding for trehalose-producing enzymes, a glycosyl-trehalose-producing enzyme (glycosyltransferase) and a glycosyl-trehalose-hydrolyzing enzyme (alpha-amylase), from Sulfolobus sol-fataricus KM1 were cloned and expressed in E. coli. The nucleotide sequence of the glycosyltransferase gene and the alpha-amylase gene indicated proteins with lengths of 728 and 558 amino acids and molecular masses of 86-kDa and 65-kDa, respectively. Regions highly conserved in the alpha-amylasefamily exist in the amino acid sequences of these enzymes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 02:22:15