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Titolo:
GENE CLONING AND EXPRESSION OF NEW TREHALOSE-PRODUCING ENZYMES FROM THE HYPERTHERMOPHILIC ARCHAEUM SULFOLOBUS-SOLFATARICUS KM1
Autore:
KOBAYASHI K; KATO M; MIURA Y; KETTOKU M; KOMEDA T; IWAMATSU A;
Indirizzi:
KIRIN BREWERY CO LTD,APPL BIORES CTR,3 MIYAHARA CHO TAKASAKI GUMMA 37012 JAPAN KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,KANAZAWA KU YOKOHAMA KANAGAWA 236 JAPAN
Titolo Testata:
Bioscience, biotechnology, and biochemistry
fascicolo: 11, volume: 60, anno: 1996,
pagine: 1882 - 1885
SICI:
0916-8451(1996)60:11<1882:GCAEON>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARCHAEBACTERIUM PYROCOCCUS-FURIOSUS; THERMOSTABLE ALPHA-AMYLASE;
Keywords:
TREHALOSE; SULFOLOBUS; GLYCOSYLTRANSFERASE; GLYCOSYLTREHALOSE; ALPHA-AMYLASE FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
K. Kobayashi et al., "GENE CLONING AND EXPRESSION OF NEW TREHALOSE-PRODUCING ENZYMES FROM THE HYPERTHERMOPHILIC ARCHAEUM SULFOLOBUS-SOLFATARICUS KM1", Bioscience, biotechnology, and biochemistry, 60(11), 1996, pp. 1882-1885

Abstract

The genes encoding for trehalose-producing enzymes, a glycosyl-trehalose-producing enzyme (glycosyltransferase) and a glycosyl-trehalose-hydrolyzing enzyme (alpha-amylase), from Sulfolobus sol-fataricus KM1 were cloned and expressed in E. coli. The nucleotide sequence of the glycosyltransferase gene and the alpha-amylase gene indicated proteins with lengths of 728 and 558 amino acids and molecular masses of 86-kDa and 65-kDa, respectively. Regions highly conserved in the alpha-amylasefamily exist in the amino acid sequences of these enzymes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 02:22:15