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Titolo:
THE EFFECT OF MILD TRYPSIN DIGESTION OF F1 ON ENERGY COUPLING IN THE MITOCHONDRIAL ATP SYNTHASE
Autore:
XU T; CANDITA C; PAPA S;
Indirizzi:
UNIV BARI,INST MED BIOCHEM & CHEM,PIAZZA G CESARE I-70124 BARI ITALY UNIV BARI,INST MED BIOCHEM & CHEM I-70124 BARI ITALY ACAD SINICA,INST BIOPHYS BEIJING 100080 PEOPLES R CHINA
Titolo Testata:
FEBS letters
fascicolo: 2-3, volume: 397, anno: 1996,
pagine: 308 - 312
SICI:
0014-5793(1996)397:2-3<308:TEOMTD>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOVINE HEART-MITOCHONDRIA; ADENOSINE-TRIPHOSPHATASE F1; TIGHTLY BOUND NUCLEOTIDES; CONFERRING PROTEIN OSCP; ESCHERICHIA-COLI; SUBMITOCHONDRIAL PARTICLES; GAMMA-SUBUNIT; H+-ATPASE; F1-ATPASE; RECONSTITUTION;
Keywords:
ATP SYNTHASE; F1; TRYPSIN DIGESTION; ENERGY COUPLING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
T. Xu et al., "THE EFFECT OF MILD TRYPSIN DIGESTION OF F1 ON ENERGY COUPLING IN THE MITOCHONDRIAL ATP SYNTHASE", FEBS letters, 397(2-3), 1996, pp. 308-312

Abstract

Mild trypsin digestion of isolated bovine-heart mitochondrial F-1-ATPase removed the first 15 residues from the N-terminus of subunit alphaunder conditions in which other F-1 subunits were apparently untouched. When the trypsinized F-1 (TF1) was reconstituted with the F-0 sector in the mitochondrial membrane (USMP), the ATP hydrolase activity acquired oligomycin sensitivity but ATP hydrolysis was decoupled from proton pumping. TF1 added to USMP did not block the proton channel in F-0as the native F-1 did. AMP-PNP inhibited proton conductivity in reconstituted F-1-USMP but this effect was lost in reconstituted TF1-USMP. These results indicate that the N-terminus of the F-1 alpha subunit plays a critical role in the conformational communication between F-1 and F-0.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 15:31:43