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Titolo:
IDENTIFICATION OF AMINO-ACID-RESIDUES REQUIRED FOR A SPECIFIC INTERACTION BETWEEN SRC-TYROSINE KINASE AND PROLINE-RICH REGION OF PHOSPHATIDYLINOSITOL-3' KINASE
Autore:
MAK P; HE ZQ; KUROSAKI T;
Indirizzi:
WYETH AYERST RES,MOL BIOL SECT,401 N MIDDLETOWN RD,B205 PEARL RIVER NY 10965
Titolo Testata:
FEBS letters
fascicolo: 2-3, volume: 397, anno: 1996,
pagine: 183 - 185
SICI:
0014-5793(1996)397:2-3<183:IOARFA>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYMPHOCYTES-B; PROTEIN; ACTIVATION; BINDING; EXPRESSION; RECEPTOR; 3-KINASE; CLONING; SUBUNIT; SIGNAL;
Keywords:
FYN; PHOSPHATIDYLINOSITOL-3' KINASE; YEAST, 2 HYBRID; SITE-DIRECTED MUTAGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
P. Mak et al., "IDENTIFICATION OF AMINO-ACID-RESIDUES REQUIRED FOR A SPECIFIC INTERACTION BETWEEN SRC-TYROSINE KINASE AND PROLINE-RICH REGION OF PHOSPHATIDYLINOSITOL-3' KINASE", FEBS letters, 397(2-3), 1996, pp. 183-185

Abstract

The binding of ligand to B-cell antigen receptors (BCR) leads to the activation of receptor-associated Src-family kinases and phosphatidylinositol-3' kinase (PI-3 kinase), Although it has been demonstrated that SH3 domains of several Src-family kinases interact with PI-3 kinase by binding to a proline-rich region of PI-3 kinase in vitro, there is no direct evidence to support their interaction in vivo, Thus, we utilized the yeast two-hybrid assay to reconstitute this protein-protein interaction, This genetic screen clearly indicates that the interactionbetween SH3 domain of Fyn and the proline-rich region (residues: 80-104) of PI-3 kinase is' highly specific, Mutational analysis revealed that amino acid residues Asp(92), Tyr(93), Arg(96) and Thr(97) of the SH3 domain of Fyn are essential for interacting with the proline-rich peptide of PI-3 kinase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 21:23:23