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Titolo:
INDUCTION OF A PHYSICAL LINKAGE BETWEEN INTEGRINS AND THE CYTOSKELETON DEPENDS ON INTRACELLULAR CALCIUM IN AN EPITHELIAL-CELL LINE
Autore:
NEBE B; BOHN W; SANFTLEBEN H; RYCHLY J;
Indirizzi:
UNIV ROSTOCK,INNERE MED KLIN,DEPT INTERNAL MED,ERNST HEYDEMANN STR D-18055 ROSTOCK GERMANY UNIV ROSTOCK,INNERE MED KLIN,DEPT INTERNAL MED D-18055 ROSTOCK GERMANY UNIV HAMBURG,HEINRICH PETTE INST EXPT VIROL & IMMUNOL D-2000 HAMBURG GERMANY
Titolo Testata:
Experimental cell research
fascicolo: 1, volume: 229, anno: 1996,
pagine: 100 - 110
SICI:
0014-4827(1996)229:1<100:IOAPLB>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
NORMAL HUMAN KERATINOCYTES; FOCAL ADHESION KINASE; EXTRACELLULAR-MATRIX; SIGNAL-TRANSDUCTION; SURFACE POLARITY; TERMINAL DIFFERENTIATION; CYTOPLASMIC DOMAIN; ENDOTHELIAL-CELLS; GENE-EXPRESSION; FIBRONECTIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
B. Nebe et al., "INDUCTION OF A PHYSICAL LINKAGE BETWEEN INTEGRINS AND THE CYTOSKELETON DEPENDS ON INTRACELLULAR CALCIUM IN AN EPITHELIAL-CELL LINE", Experimental cell research, 229(1), 1996, pp. 100-110

Abstract

In most cases epithelial cells reveal a polarized distribution of integrin receptors in basolateral domains of the plasma membrane. To evaluate the functional state of integrin receptors in these restricted sites we were interested in the physical association of integrins with the cytoskeleton. Basically, we extracted cells with Triton X-100 to obtain detergent insoluble cytoskeleton fractions and used monoclonal antibodies for the detection of integrins linked to the cytoskeleton. Wefound that no permanent physical integrin-cytoskeleton associations exist in a confluent culture of the hepatocyte cell line mHepR1. However, incubation with anti-integrin antibodies and cross linking with a secondary antibody induced a physical linkage of beta 1 as well as of different alpha subunits to the cytoskeleton. The association of integrins with the cytoskeleton was also inducible in suspended cells, whichwas detected in how cytometric analyses and indicates that the formation of a physical integrin-cytoskeleton connection is independent of the localization of integrins, cell shape, and adhesion on a substrate. Using the Ca2+ chelators BAPTA-AM and EGTA, we found that intracellular calcium is a necessary prerequisite to induce a connection of integrins to the cytoskeleton. ATP or tauroursodeoxycholic acid, which provoke an intracellular calcium elevation, partly induced the formation of an integrin-cytoskeleton linkage. These results indicate the obviousrole of intracellular calcium in integrin-dependent outside-in as well as inside-out signaling. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 18:11:56