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Titolo:
SYNTHESIS AND CHARACTERIZATION OF AN ACID PHOSPHATASE-POLYRESORCINOL COMPLEX
Autore:
GARZILLO AMV; BADALUCCO L; DECESARE F; GREGO S; BUONOCORE V;
Indirizzi:
UNIV TUSCIA,DIPARTIMENTO AGROBIOL & AGROCHIM,VIA S CAMILLA DE LELLIS I-01100 VITERBO ITALY
Titolo Testata:
Soil biology & biochemistry
fascicolo: 9, volume: 28, anno: 1996,
pagine: 1155 - 1161
SICI:
0038-0717(1996)28:9<1155:SACOAA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMIC-ENZYME COMPLEXES; PHENOLIC COPOLYMERS; ORGANIC PHOSPHORUS; SOIL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
A.M.V. Garzillo et al., "SYNTHESIS AND CHARACTERIZATION OF AN ACID PHOSPHATASE-POLYRESORCINOL COMPLEX", Soil biology & biochemistry, 28(9), 1996, pp. 1155-1161

Abstract

A stable acid phosphatase-polyresorcinol complex, retaining about 90%of the original enzymatic activity, was synthesized in the presence of peroxidase. The complex was disturbed by chaotropic agents, as 4 M urea, indicating that the enzyme linked the phenolic moiety by non-covalent bonds. The immobilized phosphatase was more stable than the free enzyme towards pH, temperature and proteolysis; however, when added tosoil, free and immobilized phosphatase showed comparable stability. It appears that the polymerization conditions used, favouring the interaction between enzyme and polyphenol through secondary linkages, give rise to a fully active complex resembling those which naturally generate in soil. Copyright (C) 1996 Elsevier Science Ltd

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 08:32:47