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Titolo:
MUSCLE DIFFERENTIATION DURING REPAIR OF MYOCARDIAL NECROSIS IN RATS VIA GENE-TRANSFER WITH MYOD
Autore:
MURRY CE; KAY MA; BARTOSEK T; HAUSCHKA SD; SCHWARTZ SM;
Indirizzi:
UNIV WASHINGTON,DEPT PATHOL,00000BOX 357470,ROOM E520,HSB SEATTLE WA 98195 UNIV WASHINGTON,DEPT MED,DIV MED GENET SEATTLE WA 98195 UNIV WASHINGTON,DEPT BIOCHEM SEATTLE WA 98195
Titolo Testata:
The Journal of clinical investigation
fascicolo: 10, volume: 98, anno: 1996,
pagine: 2209 - 2217
SICI:
0021-9738(1996)98:10<2209:MDDROM>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYOSIN HEAVY-CHAIN; SKELETAL-MUSCLE; TERMINAL DIFFERENTIATION; MYOGENIC LINEAGE; IN-VIVO; EXPRESSION; CELLS; ACTIVATION; HEART; FIBROBLASTS;
Keywords:
MYOCARDIAL INFARCTION; MYOGENIC DETERMINATION FACTOR; MYOD; REGENERATION; MYOCARDIAL INFARCT REPAIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
C.E. Murry et al., "MUSCLE DIFFERENTIATION DURING REPAIR OF MYOCARDIAL NECROSIS IN RATS VIA GENE-TRANSFER WITH MYOD", The Journal of clinical investigation, 98(10), 1996, pp. 2209-2217

Abstract

Myocardial infarcts heal by scar formation because there are no stem cells in myocardium, and because adult myocytes cannot divide and repopulate the wound, We sought to redirect the heart to form skeletal muscle instead of scar by transferring the myogenic determination gene, MyoD, into cardiac granulation (wound repair) tissue, A replication-defective adenovirus was constructed containing MyoD under transcriptional control of the Rous sarcoma virus long terminal repeat, The virus converted cultured cardiac fibroblasts to skeletal muscle, indicated by expression of myogenin and skeletal myosin heavy chains (MHCs). To determine if MyoD could induce muscle differentiation in vivo, we injected 2 x 10(9) or 10(10) pfu of either the MyoD or a control beta-galactosidase adenovirus into healing rat hearts, injured 1 wk previously by freeze-thaw, After receiving the lower viral dose, cardiac granulationtissue expressed MyoD mRNA and protein, but did not express myogenin or skeletal MHC. When the higher dose of virus was administered, double immunostaining showed that cells in reparative tissue expressed bothmyogenin and embryonic skeletal MHC, No muscle differentiation occurred after beta-galactosidase transfection, Thus, MyoD gene transfer caninduce skeletal muscle differentiation in healing heart lesions, Modifications of this strategy might eventually provide new contractile tissue to repair myocardial infarcts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 14:12:07