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Titolo:
COMBINATION OF ANTIVIRAL IMMUNOTOXIN AND GANCICLOVIR OR CIDOFOVIR FORTHE TREATMENT OF MURINE CYTOMEGALOVIRUS INFECTIONS
Autore:
SMEE DF; SIDWELL RW; BARNETT BB;
Indirizzi:
UTAH STATE UNIV,INST ANTIVIRAL RES LOGAN UT 84322
Titolo Testata:
Antiviral research
fascicolo: 3, volume: 32, anno: 1996,
pagine: 165 - 171
SICI:
0166-3542(1996)32:3<165:COAIAG>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMBINED IMMUNODEFICIENT MICE; CELL-CULTURE; VIRUS; ANTIBODY; -1-)3-HYDROXY-2-(PHOSPHONYLMETHOXY)PROPYL>CYTOSINE; ACYCLOVIR; INVITRO;
Keywords:
IMMUNOTOXIN; GANCICLOVIR; CIDOFOVIR; SCID MICE; CYTOMEGALOVIRUS; ANTIVIRAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
D.F. Smee et al., "COMBINATION OF ANTIVIRAL IMMUNOTOXIN AND GANCICLOVIR OR CIDOFOVIR FORTHE TREATMENT OF MURINE CYTOMEGALOVIRUS INFECTIONS", Antiviral research, 32(3), 1996, pp. 165-171

Abstract

The effects of two anti-murine cytomegalovirus (MCMV) immunotoxins used in combination with ganciclovir (GVC) or cidofovir (HPMPC) against MCMV were determined in vitro and in mice. The inhibitors were added to cell cultures 24 or 48 h after MCMV adsorption so as to not affect the initial infection rate. The immunotoxins (0.63, 1.25 and 2.5 mu g/ml) combined with GCV (1.25, 2.5 and 5 mu M) or HPMPC (0.03, 0.06 and 0.12 mu M) caused synergistic inhibition of virus yield in C127I cells at most of the combinations tested. No toxic effect on cell growth in culture was observed at these immunotoxin/drug combinations. The effects of immunotoxin and GCV treatment were studied further in MCMV-infected severe combined immunodeficient (SCID) mice. Immunotoxin (1 mg/kg per day) given by intraperitoneal (i.p.) injection on days 1, 4 and 7 of the infection did not extend the mean day to death compared with the placebo group. Once daily i.p. treatment with GCV (50 mg/kg per day)for 7 days starting at 24 h after virus inoculation extended survivaltime almost 11 days. The combination of immunotoxin plus GCV was better than GCV alone, extending the mean day to death an additional 2 to 3 days, which is suggestive of a synergistic effect.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 07:36:38