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Titolo:
PROKARYOTIC DNA-SEQUENCES IN PATIENTS WITH CHRONIC IDIOPATHIC PROSTATITIS
Autore:
KRIEGER JN; RILEY DE; ROBERTS MC; BERGER RE;
Indirizzi:
UNIV WASHINGTON,SCH MED,DEPT UROL,BOX 356510 SEATTLE WA 98195 UNIV WASHINGTON,SCH PUBL HLTH & COMMUNITY MED,DEPT PATHOBIOL SEATTLE WA 98195
Titolo Testata:
Journal of clinical microbiology
fascicolo: 12, volume: 34, anno: 1996,
pagine: 3120 - 3128
SICI:
0095-1137(1996)34:12<3120:PDIPWC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; CHRONIC ABACTERIAL PROSTATITIS; HERPES-SIMPLEX VIRUS; 16S RIBOSOMAL-RNA; CHLAMYDIA-TRACHOMATIS; UREAPLASMA-UREALYTICUM; TRICHOMONAS-VAGINALIS; IDENTIFICATION; MEN; MICROORGANISMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
62
Recensione:
Indirizzi per estratti:
Citazione:
J.N. Krieger et al., "PROKARYOTIC DNA-SEQUENCES IN PATIENTS WITH CHRONIC IDIOPATHIC PROSTATITIS", Journal of clinical microbiology, 34(12), 1996, pp. 3120-3128

Abstract

Half of all men experience symptoms of prostatitis at some time in their lives, but the etiology is unknown for more than 90% of patients. Optimal clinical and culture methods were used to select 135 men with chronic prostatitis refractory to multiple previous courses of antimicrobial therapy. The subjects had no evidence of structural or functional lower genitourinary tract abnormalities of bacteriuria or bacterialprostatitis by traditional clinical tests, or of urethritis or urethral pathogens by culture. Specific PCR assays detected Mycoplasma genitalium, Chlamydia trachomatis, or Trichomonas vaginalis in 10 patients (8%). Broad-spectrum PCR tests detected tetracycline resistance-encoding genes, tetM-tetO-tetS, in 25% of patients and 16S rRNA in 77% of subjects. The tetM-tetO-tetS-positive cases constituted a subset of the 16S rRNA-positive cases. Patients with 16S rRNA were more likely to have greater than or equal to 1,000 leukocytes per mm(3) in their expressed prostatic secretions than men whose prostate biopsy specimens merenegative for 16S rRNA (P < 0.001). Based on direct sequencing and repetitive cloning, multiple sources of 16S rRNA were observed in individual patients, Sequences of 29 cloned PCR products revealed 16S rRNAs distinct from those of common skin and gut flora. These findings suggest that the prostate can harbor microorganisms that are not detectable by traditional approaches. These organisms may be associated with inflammation in the expressed prostatic secretions. Molecular methods holdgreat promise for identifying culture-resistant microorganisms in patients with chronic prostatitis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 21:13:58