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Titolo:
PRESYNAPTIC MODULATION BY VIP, SECRETIN AND ISOPROTERENOL OF SOMATOSTATIN RELEASE FROM ENRICHED ENTERIC SYNAPTOSOMES - ROLE OF CAMP
Autore:
KURJAK M; SCHUSDZIARRA V; ALLESCHER HD;
Indirizzi:
TECH UNIV MUNICH,DEPT INTERNAL MED 2,ISMANINGER STR 22 D-81675 MUNICHGERMANY TECH UNIV MUNICH,DEPT INTERNAL MED 2 D-81675 MUNICH GERMANY
Titolo Testata:
European journal of pharmacology
fascicolo: 1-2, volume: 314, anno: 1996,
pagine: 165 - 173
SICI:
0014-2999(1996)314:1-2<165:PMBVSA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASOACTIVE INTESTINAL POLYPEPTIDE; PIG SMALL-INTESTINE; PERFUSED RAT STOMACH; BOMBESIN-LIKE IMMUNOREACTIVITY; CEREBRAL CORTICAL-CELLS; CANINE SMALL-INTESTINE; MYENTERIC PLEXUS; CYCLIC-AMP; ACETYLCHOLINE-RELEASE; DIENCEPHALIC CELLS;
Keywords:
SYNAPTOSOME; VIP (VASOACTIVE INTESTINAL POLYPEPTIDE); SECRETIN; ISOPROTERENOL; CAMP; NITRIC OXIDE (NO);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
M. Kurjak et al., "PRESYNAPTIC MODULATION BY VIP, SECRETIN AND ISOPROTERENOL OF SOMATOSTATIN RELEASE FROM ENRICHED ENTERIC SYNAPTOSOMES - ROLE OF CAMP", European journal of pharmacology, 314(1-2), 1996, pp. 165-173

Abstract

The release of somatostatin-like immunoreactivity was studied in isolated enteric synaptosomes. A significant release of somatostatinlike immunoreactivity was observed in the presence of vasoactive intestinal polypeptide (VIP) (10(-6) M: 53.0 +/- 12.4 pg/mg, basal: 14.3 +/- 1.7 pg/mg, n = 5, P < 0.05), secretin(10(-6) M: 56.1 +/- 3.8 pg/mg, basal:25.8 +/- 1.6 pg/mg, n = 6, P < 0.01) and isoproterenol (10(-5) M: 54.0 +/- 13.4 pg/mg, basal: 20.0 +/- 3.4 pg/mg, n = 8, P < 0.05). Forskolin, an unspecific activator of the adenylate cyclase, caused a significant release of somatostatin-like immunoreactivity (10(-6) M: 57.3 +/-13.2 pg/mg, basal: 30.0 +/- 5.8 pg/mg, n = 13, P < 0.01) which was further augmented in the presence of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX 10(-4) M) (77.0 +/- 17.8 pg/mg, n = 13,P < 0.01). 3-Isobutyl-1-methylxanthine and N-6,2'-O-dibutyryladenosine-3',5'-cyclic monophosphate mimicked the effect of forskolin and VIP. The release of somatostatin was paralleled by an increase of cAMP immunoreactivity in the presence of VIP (10(-6) M: 37.1 +/- 9.4 pmol/mg, basal: 19.8 +/- 4.2 pmol/mg, n = 10, P < 0.05), isoproterenol (10(-5) M: 42.4 +/- 9.8 pmol/mg, basal: 16.7 +/- 2.4 pmol/mg, n = 12, P < 0.01) and forskolin (10(-6) M: 47.1 +/- 12.4 pmol/mg, basal: 19.8 +/- 4.2 pmol/mg, n = 10, P < 0.01). The effect of nitric oxide (NO) which actsas an inhibitory neurotransmitter in the enteric nervous system was studied. NO is known to activate soluble guanylate cyclase to induce transmitter release. The NO-generating compound sodium nitroprusside andbromoguanosine-3',5'-cyclic monophosphate (8-Br-cGMP) had no effect on the release of somatostatin-like immunoreactivity. These data demonstrate the stimulatory effect of VIP, secretin and isoproterenol on release of somatostatin-like immunoreactivity from enteric synaptosomes, which is presumably mediated by cAMP-dependent mechanisms. cGMP-dependent mechanisms seem to be of minor relevance.

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Documento generato il 04/12/20 alle ore 00:14:05