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Titolo:
APPLICATION OF A CHAIN TERMINATION ASSAY FOR CHARACTERIZATION OF REVERSE-TRANSCRIPTASE FROM AZT-RESISTANT HIV ISOLATES
Autore:
LENNERSTRAND J; RYTTING AS; VRANG L; GRONOWITZ JS; KALLANDER CFR;
Indirizzi:
UNIV UPPSALA,CTR BIOMED,DEPT MED GENET,RES UNIT REPLICAT ENZYMOL,BOX 584 S-75123 UPPSALA SWEDEN UNIV UPPSALA,CTR BIOMED,DEPT MED GENET,RES UNIT REPLICAT ENZYMOL S-75123 UPPSALA SWEDEN SANGTEC MED AB S-16120 BROMMA SWEDEN MEDIVIR AB S-14144 HUDDINGE SWEDEN
Titolo Testata:
Antiviral chemistry & chemotherapy
fascicolo: 6, volume: 7, anno: 1996,
pagine: 313 - 320
SICI:
0956-3202(1996)7:6<313:AOACTA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; AZIDOTHYMIDINE TRIPHOSPHATE; RNASE-H; ZIDOVUDINE; 3'-AZIDO-3'-DEOXYTHYMIDINE; PURIFICATION; SENSITIVITY; HETERODIMER; INHIBITION; MUTATIONS;
Keywords:
ANTIVIRAL SUBSTANCES; AZT, CHAIN TERMINATION; ENZYME-ASSAYS; HIV/AIDS; REVERSE TRANSCRIPTASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
J. Lennerstrand et al., "APPLICATION OF A CHAIN TERMINATION ASSAY FOR CHARACTERIZATION OF REVERSE-TRANSCRIPTASE FROM AZT-RESISTANT HIV ISOLATES", Antiviral chemistry & chemotherapy, 7(6), 1996, pp. 313-320

Abstract

An enzymatic assay based on utilization of one primer/enzyme moleculewas specifically designed for evaluation of the chain termination capacity of reverse transcriptase (RT) from HIV-1 isolates. In this assaysystem (CT assay) there was a 3.2-fold difference between the AZT-triphosphate (AZT-TP) concentrations required to terminate 50% of the primers (TC50) for a highly resistant isolate, carrying the four common mutations at positions 67, 70, 215, and 219; and two wild type isolates. Two of three other isolates with reduced sensitivity to AZT in cell culture exhibited intermediate values in CT assay, while one behaved as the wild type isolates. There was a correlation P = 0.05 (r = 0.86, n = 6) between the ED(50) values found in cell culture and the TC50 values found in CT assay. This relationship was not found in a similar assay system which measured competition between AZT-TP and 1 mu M tymidine triphosphate (TTP) at the enzymatic level. The sequence data of the current isolates gave some information concerning which mutations inthe RT gene specifically affect the enzymatic properties measured in CT assay. Mutation only at amino acid 70 had no effect, but the TC50 values found increased with accumulation of the other common AZT resistance mutations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:56:08