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Titolo:
A DIFFERENTIAL ROLE FOR CELL-SHAPE IN NEUTROPHIL TETHERING AND ROLLING ON ENDOTHELIAL SELECTINS UNDER FLOW
Autore:
FINGER EB; BRUEHL RE; BAINTON DF; SPRINGER TA;
Indirizzi:
HARVARD UNIV,SCH MED,CTR BLOOD RES,200 LONGWOOD AVE BOSTON MA 02115 HARVARD UNIV,SCH MED,CTR BLOOD RES BOSTON MA 02115 HARVARD UNIV,SCH MED,DEPT PATHOL BOSTON MA 02115 UNIV CALIF SAN FRANCISCO,DEPT PATHOL SAN FRANCISCO CA 94143
Titolo Testata:
The Journal of immunology
fascicolo: 11, volume: 157, anno: 1996,
pagine: 5085 - 5096
SICI:
0022-1767(1996)157:11<5085:ADRFCI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
NODE HOMING RECEPTOR; LEUKOCYTE ADHESION MOLECULE-1; P-SELECTIN; PHYSIOLOGICAL FLOW; SPATIAL-DISTRIBUTION; CHEMOTACTIC FACTORS; CYTOPLASMIC DOMAIN; GMP-140 BINDS; LIGAND; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
E.B. Finger et al., "A DIFFERENTIAL ROLE FOR CELL-SHAPE IN NEUTROPHIL TETHERING AND ROLLING ON ENDOTHELIAL SELECTINS UNDER FLOW", The Journal of immunology, 157(11), 1996, pp. 5085-5096

Abstract

We investigated the role of neutrophil microvilli in interactions with E-selectin and P-selectin in hydrodynamic shear flow by disruption with cytochalasin B, hypotonic swelling, and chilling. Cytochalasin B only marginally reduced microvilli numbers (from 30 +/- 6 to 16 +/- 6 per cell perimeter, p < 0.005) as shown by electron microscopy, completely disrupted tethering in shear flow to E-sesectin and P-selectin, increased the strength of rolling adhesions on E-selectin and P-selectin, and increased cell deformability in shear flow with a likely increase in the area of cell:substrate contact. Hypoosmotic swelling markedlyreduced microvilli number (to 6 +/- 5 per perimeter, p < 0.005), almost completely inhibited tethering on E- and P-selectin, and increased the strength of rolling adhesions on P-selectin but not on E-selectin. Chilling almost completely abolished microvilli (to 3 +/- 3 per perimeter, p < 0.005), but pseudopod-like structures were present, and had little effect on tethering in flow. Immunogold labeling of L-selectin,which is normally clustered on tips of microvilli, showed that in theabsence of microvilli it remained in small clusters. Our studies showthat alterations in cell morphology and viscoelasticity can have opposing effects on tethering and rolling, showing that they are independently regulatable. Furthermore, our results suggest that the association of molecules that mediate rolling with microvilli tips may be important not just to enhance presentation, but for other functions such as to promote resistance to extraction from the membrane or cooperative interactions among clustered receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:22:05