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Titolo:
CELL-WALL ESTERIFIED PHENOLIC DIMERS - IDENTIFICATION AND QUANTIFICATION BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND DIODE-ARRAY DETECTION
Autore:
WALDRON KW; PARR AJ; NG A; RALPH J;
Indirizzi:
AFRC,INST FOOD RES,DEPT FOOD BIOPHYS,NORWICH RES PK,COLNEY LANE NORWICH NR4 7UA NORFOLK ENGLAND USDA ARS,US DAIRY FORAGE RES CTR MADISON WI 53706
Titolo Testata:
Phytochemical analysis
fascicolo: 6, volume: 7, anno: 1996,
pagine: 305 - 312
SICI:
0958-0344(1996)7:6<305:CEPD-I>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUGAR-BEET PULP; FERULOYLATED OLIGOSACCHARIDES; DIFERULIC ACID; GROWTH; COLEOPTILES; INVOLVEMENT; LIGHT;
Keywords:
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; DIODE ARRAY DETECTION; CELL WALLS; PHENOLICS; DEHYDRODIMERS; FERULIC ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
K.W. Waldron et al., "CELL-WALL ESTERIFIED PHENOLIC DIMERS - IDENTIFICATION AND QUANTIFICATION BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND DIODE-ARRAY DETECTION", Phytochemical analysis, 7(6), 1996, pp. 305-312

Abstract

An optimized high performance liquid chromatography (HPLC) procedure has been developed for the analysis and quantification of all of the known ferulic acid dehydrodimers, and the principle phenolic aldehydes and acids, found in the cell walls of higher plants. The HPLC system uses an ODS2 reverse phase column (5 mu m particle size) eluted with a methanol, acetonitrile and water gradient with detection at 280 nm. Inaddition to providing baseline resolution of most components, the method employs a spectrometric detector which enables the precise identification of eluted components through the analysis of their spectral properties, Analysis of the cell wall phenolics of wheat straw stem (Triticum vulgare) was carried out using this method which is highly versatile and, for certain components, more sensitive than the current gas chromatography-mass spectrometry methodology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 20:03:57