Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CELLULAR-RESPONSE TO TRANSFORMING GROWTH-FACTOR-BETA-1 AND BASIC FIBROBLAST GROWTH-FACTOR DEPENDS ON RELEASE KINETICS AND EXTRACELLULAR-MATRIX INTERACTIONS
Autore:
DINBERGS ID; BROWN L; EDELMAN ER;
Indirizzi:
HARVARD UNIV,MIT,DIV HLTH SCI & TECHNOL CAMBRIDGE MA 02139 BRIGHAM & WOMENS HOSP,DEPT MED,DIV CARDIOVASC BOSTON MA 02115 HARVARD UNIV,SCH MED BOSTON MA 02115
Titolo Testata:
The Journal of biological chemistry
fascicolo: 47, volume: 271, anno: 1996,
pagine: 29822 - 29829
SICI:
0021-9258(1996)271:47<29822:CTTGAB>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAPILLARY ENDOTHELIAL-CELLS; PLASMINOGEN-ACTIVATOR PRODUCTION; HEPARIN-LIKE MOLECULES; FACTOR-BETA; TGF-BETA; SUSTAINED-RELEASE; DNA-SYNTHESIS; PROTEOLYTIC DEGRADATION; ANGIOGENIC ACTIVITY; BASEMENT-MEMBRANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
95
Recensione:
Indirizzi per estratti:
Citazione:
I.D. Dinbergs et al., "CELLULAR-RESPONSE TO TRANSFORMING GROWTH-FACTOR-BETA-1 AND BASIC FIBROBLAST GROWTH-FACTOR DEPENDS ON RELEASE KINETICS AND EXTRACELLULAR-MATRIX INTERACTIONS", The Journal of biological chemistry, 271(47), 1996, pp. 29822-29829

Abstract

The extracellular matrix plays an important role in growth factor biology, serving as a potential platform for rapid growth factor mobilization or a sink for concentrated sequestration, We now demonstrate thatwhen a growth factor binds reversibly to the matrix, its effects are augmented by this interaction, and when the factor is absorbed irreversibly to the extracellular matrix, it becomes sequestered. These findings call into question the notion that all growth factors are best presented to cells and tissues in a sustained and controlled fashion, In our studies, we examined basic fibroblast growth factor (bFGF) and transforming growth factor-beta 1 (TGF-beta 1) release kinetics from synthetically fabricated microsphere devices and naturally synthesized extracellular matrix. While the sustained release of bFGF was up to 3.0-fold more potent at increasing vascular endothelial and smooth muscle cell proliferation than bolus administration, the reverse was true for TGF-beta 1 A bolus of TGF-beta 1 inhibited vascular cells up to 3.8-fold more efficiently than the same amount of TGF-beta 1 if control-released. Both growth factors bound to the extracellular matrix, but only bFGF was released in a controlled fashion (2.8%/day). Contact with theextracellular matrix and subsequent release enhanced bFGF activity such that it was 86% more effective at increasing smooth muscle cell numbers than equal amounts of growth factor diluted from frozen stock. TGF-beta 1 remained tightly adherent, The small amount of TGF-beta 1 released from the extracellular matrix was similar to 30% less effective than bolus administration at inhibiting vascular endothelial and smooth muscle cell growth, Sustained growth factor release may be the preferable mode of administration, but only when a similar mode of metabolism is utilized endogenously.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:26:06