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Titolo:
THE EFFECT OF OUABAIN (INHIBITOR OF AN ATP-DEPENDENT NA+ K+ PUMP) ON THE PANCREAS GRAFT DURING PRESERVATION BY THE 2-LAYER METHOD/
Autore:
TANIOKA Y; KURODA Y; KIM Y; MATSUMOTO S; SUZUKI Y; KU Y; FUJITA H; SAITOH Y;
Indirizzi:
KOBE UNIV,SCH MED,DEPT SURG 1,CHUO KU,7-5-2 KUSUNOKI CHO KOBE 650 JAPAN KOBE UNIV,SCH MED,DEPT SURG 1,CHUO KU KOBE 650 JAPAN
Titolo Testata:
Transplantation
fascicolo: 12, volume: 62, anno: 1996,
pagine: 1730 - 1734
SICI:
0041-1337(1996)62:12<1730:TEOO(O>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLD-STORAGE METHOD; TRIPHOSPHATE TISSUE CONCENTRATION; CANINE PANCREAS; ADENOSINE-TRIPHOSPHATE; 72-HOUR PRESERVATION; LIVER; MECHANISM; INJURY; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
Y. Tanioka et al., "THE EFFECT OF OUABAIN (INHIBITOR OF AN ATP-DEPENDENT NA+ K+ PUMP) ON THE PANCREAS GRAFT DURING PRESERVATION BY THE 2-LAYER METHOD/", Transplantation, 62(12), 1996, pp. 1730-1734

Abstract

To clarify how synthesized ATP is utilized to maintain cellular integrity during preservation by the two-layer method, we examined the effect of ouabain, inhibitor of ATP dependent Na+/K+ pump, on ATP tissue levels, graft weight, and vascular endothelial cells during 48 hr preservation by the two-layer method and pancreatic tissue perfusion and graft survival after transplantation in a canine model, Ouabain treatment did not affect ATP production but prevented its utilization by the sodium pump, and actually significantly increased ATP levels and decreased the weight loss of the graft, In addition, ouabain caused a significant increase of nuclear trypan-blue staining in vascular endothelialcells and a significant decrease of pancreatic tissue perfusion at reperfusion. Consequently, the grafts did not survive, We conclude that the two-layer method allows sufficient synthesis of ATP to drive the sodium pump and maintains membrane integrity of parenchymal cells and vascular endothelial cells, thus extending the period of preserved pancreatic viability.

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Documento generato il 02/04/20 alle ore 19:26:19