Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
GENETIC-ANALYSIS OF THE HUMAN CYTOCHROME-P450 CYP2C9 LOCUS
Autore:
STUBBINS MJ; HARRIES LW; SMITH G; TARBIT MH; WOLF CR;
Indirizzi:
UNIV DUNDEE,NINEWELLS HOSP,CTR BIOMED RES DUNDEE DD1 9SY TAYSIDE SCOTLAND UNIV DUNDEE,NINEWELLS HOSP,CTR BIOMED RES DUNDEE DD1 9SY TAYSIDE SCOTLAND GLAXO WELLCOME PLC,DRUG METAB 3 WARE SG12 0DP HERTS ENGLAND
Titolo Testata:
Pharmacogenetics
fascicolo: 5, volume: 6, anno: 1996,
pagine: 429 - 439
SICI:
0960-314X(1996)6:5<429:GOTHCC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID; (S)-MEPHENYTOIN 4'-HYDROXYLASE; POOR METABOLIZERS; LUNG-CANCER; POLYMORPHISM; SUBFAMILY; DEFECT; CDNA; IDENTIFICATION; DEBRISOQUINE;
Keywords:
CYTOCHROME P450 CYP2C9; GENETIC POLYMORPHISM; POLYMERASE CHAIN REACTION; DRUG METABOLISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
M.J. Stubbins et al., "GENETIC-ANALYSIS OF THE HUMAN CYTOCHROME-P450 CYP2C9 LOCUS", Pharmacogenetics, 6(5), 1996, pp. 429-439

Abstract

Cytochrome P450 CYP2C9 metabolizes a wide variety of clinically important drugs, including phenytoin, tolbutamide, warfarin and a large number of non-steroidal anti-inflammatory drugs. Previous studies have shown that even relatively conservative changes in the amino acid composition of this enzyme can affect both its activity and substrate specificity. To date six different human CYP2C9 cDNA sequences, as well as the highly homologous CYP2C10 sequence have been reported suggesting that the CYP2C9 gene is polymorphic. Only nine single base substitutionsin the coding region of CYP2C9 account for the differences seen between the CYP2C9 proteins. In this report we have developed polymerase chain reaction (PCR)-based assays to distinguish all seven sequences, and have determined their allele frequencies in the Caucasian population. Of the seven sequences studied in one hundred individuals only threeappeared to be CYP2C9 alleles. These alleles termed CYP2C91, CYP2C9*2 and CYP2C93 had allele frequencies of 0.79, 0.125 and 0.085 respectively. The CYP2C10 gene could not be found in any of the samples studied. The assays developed here will allow the prediction of CYP2C9 phenotype, thus identifying those individuals who may exhibit different drug for CYP2C9 substrates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/02/20 alle ore 05:48:40