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Titolo:
STRUCTURAL STUDIES OF LECITHIN-BASED AND AOT-BASED WATER-IN-OIL MICROEMULSIONS, IN THE PRESENCE OF LIPASE
Autore:
AVRAMIOTIS S; STAMATIS H; KOLISIS FN; LIANOS P; XENAKIS A;
Indirizzi:
NATL HELLEN RES FDN,INST BIOL RES & BIOTECHNOL,IND ENZYMOL UNIT,48 VAS CONSTANTINOU AVE GR-11635 ATHENS GREECE NATL HELLEN RES FDN,INST BIOL RES & BIOTECHNOL,IND ENZYMOL UNIT GR-11635 ATHENS GREECE NATL TECH UNIV ATHENS,DEPT CHEM ENGN,DIV 4 GR-15700 ATHENS GREECE UNIV PATRAS,DEPT ENGN SCI GR-26500 PATRAS GREECE
Titolo Testata:
Langmuir
fascicolo: 26, volume: 12, anno: 1996,
pagine: 6320 - 6325
SICI:
0743-7463(1996)12:26<6320:SSOLAA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE ENERGY-TRANSFER; PSEUDOMONAS-CEPACIA LIPASE; REVERSE MICELLES; ESTERIFICATION REACTIONS; W/O MICROEMULSIONS; LOCALIZATION; BEHAVIOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
S. Avramiotis et al., "STRUCTURAL STUDIES OF LECITHIN-BASED AND AOT-BASED WATER-IN-OIL MICROEMULSIONS, IN THE PRESENCE OF LIPASE", Langmuir, 12(26), 1996, pp. 6320-6325

Abstract

Water-in-oil microemulsions based on lecithin or AOT were used to solubilized lipase from Pseudomonas cepacia. The enzyme activity was tested in the catalysis of an esterification reaction between lauric acid and various short chained aliphatic alcohols. Lipase was found to be more specific for propanol in the lecithin-based system, while in the AOT microemulsions the enzyme did not show any alcohol preference. Fluorescence energy transfer measurements were applied to detect the site of localization of the enzyme molecule within the different domains ofthe microemulsion droplets. In both cases the enzyme was found to be close to the surfactant interface, with the efficiency of the transferbeing more pronounced in the AOT systems. Time-resolved luminescence quenching was also applied to both systems, to probe structural modifications of the microemulsions induced by the presence of lipase. Communication between the dispersed aqueous microdomains, as expressed by the observation of percolative phenomena, was found to be affected.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:17:03