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Titolo:
THE CENTRAL Z-DISK REGION OF TITIN IS ASSEMBLED FROM A NOVEL REPEAT IN VARIABLE COPY NUMBERS
Autore:
GAUTEL M; GOULDING D; BULLARD B; WEBER K; FURST DO;
Indirizzi:
EUROPEAN MOL BIOL LAB,DIV STRUCT BIOL HEIDELBERG GERMANY MAX PLANCK INST BIOPHYS CHEM,DEPT BIOCHEM D-3400 GOTTINGEN GERMANY UNIV POTSDAM,DEPT CELL BIOL POTSDAM GERMANY
Titolo Testata:
Journal of Cell Science
, volume: 109, anno: 1996,
parte:, 11
pagine: 2747 - 2754
SICI:
0021-9533(1996)109:<2747:TCZROT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNOGLOBULIN-TYPE DOMAINS; SKELETAL-MUSCLE; Z-LINE; MONOCLONAL-ANTIBODIES; IMMUNOELECTRON MICROSCOPY; CONNECTIN FILAMENTS; SARCOMERE MATRIX; ELASTIC PROTEIN; GIANT; NEBULIN;
Keywords:
TITIN; CONNECTIN; Z-DISK; SARCOMERE; ISOFORM; PHOSPHORYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
M. Gautel et al., "THE CENTRAL Z-DISK REGION OF TITIN IS ASSEMBLED FROM A NOVEL REPEAT IN VARIABLE COPY NUMBERS", Journal of Cell Science, 109, 1996, pp. 2747-2754

Abstract

The giant sarcomeric protein titin (also described as connectin) is composed mainly of immunoglobulin (Ig)like and fibronectin type III (fn3)-like domains arranged consecutively, At both ends of the molecule, these domains are interrupted by sequence insertions, The amino terminus of titin is localized in the Z-disk. a structure of great variability in different muscle types. We have determined the ultrastructural position of sequences in this region of the molecule in skeletal and cardiac muscle by immunoelectron microscopy using antibodies directed against unique epitopes. Titin molecules entering the Z-disk from two half sarcomeres do not significantly overlap, showing that the amino terminus is at the centre of the Z-disk. A serine/proline rich site, which can be phosphorylated by kinases in developing muscle tissues, was identified near the amino terminus of titin. Sequence analysis revealedthe presence of a novel 45 residue repeat ('Z-repeats') in this region of the molecule, The number of titin Z-repeats varies due to differential splicing, We propose that this mechanism is a means of assembling Z-disks of variable thickness and mechanical strength.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:55:58