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Titolo:
EXPRESSION AND BIOLOGICAL-ACTIVITY OF GENETIC FUSIONS BETWEEN MALE, THE MALTOSE-BINDING PROTEIN FROM ESCHERICHIA-COLI AND PORTIONS OF CD4, THE T-CELL RECEPTOR OF THE AIDS VIRUS
Autore:
CLEMENT JM; JEHANNO M; POPESCU O; SAURIN W; HOFNUNG M;
Indirizzi:
INST PASTEUR,UNITE PROGRAMMAT MOL & TOXICOL GENET,CNRS UA1444,25 RUE DR ROUX F-75724 PARIS 15 FRANCE
Titolo Testata:
Protein expression and purification
fascicolo: 3, volume: 8, anno: 1996,
pagine: 319 - 331
SICI:
1046-5928(1996)8:3<319:EABOGF>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; RECOMBINANT SOLUBLE CD4; OUTER-MEMBRANE PROTEASE; HYBRID PROTEIN; HIV-INFECTION; ONE-STEP; TYPE-1; PURIFICATION; TRANSPORT; FRAGMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Clement et al., "EXPRESSION AND BIOLOGICAL-ACTIVITY OF GENETIC FUSIONS BETWEEN MALE, THE MALTOSE-BINDING PROTEIN FROM ESCHERICHIA-COLI AND PORTIONS OF CD4, THE T-CELL RECEPTOR OF THE AIDS VIRUS", Protein expression and purification, 8(3), 1996, pp. 319-331

Abstract

Hybrid molecules between MalE, the periplasmic maltose binding protein of Escherichia coli, and CD4, the human T-lymphocyte receptor for the AIDS virus HIV, have been constructed and purified. We show that CD4can be fused as multiple repeats to both ends of a single MalE molecule. Hybrid proteins are exported into the periplasm of bacteria, bind monoclonal antibodies directed against CD4, bind HIV gp160, and inhibit HIV binding to CD4(+) cells. MalE has been used as a scaffold to graft portions of CD4. Deletion analysis allowed to define a minimal structural domain which folds in a way which is compatible with its biological activity. This minimal part was used to design compact hybrid molecules in which CD4 was inserted internally into MalE. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 09:53:13