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Titolo:
APOPTOSIS OF L929 CELLS BY ETOPOSIDE - A QUANTITATIVE AND KINETIC APPROACH
Autore:
BONELLI G; SACCHI MC; BARBIERO G; DURANTI F; GOGLIO G; DICANTOGNO LV; AMENTA JS; PIACENTINI M; TACCHETTI C; BACCINO FM;
Indirizzi:
UNIV TURIN,DIPARTIMENTO MED & ONCOL SPERIMENTALE,CORSO RAFFAELLO 30 I-10125 TURIN ITALY UNIV PITTSBURGH,SCH MED,DEPT PATHOL PITTSBURGH PA 15260 UNIV ROMA TOR VERGATA,DIPARTIMENTO BIOL I-00173 ROME ITALY UNIV GENOA,IST ANAT I-16126 GENOA ITALY CNR,CTR IMMUNOGENET & ONCOL SPERIMENTALE I-10126 TURIN ITALY
Titolo Testata:
Experimental cell research
fascicolo: 2, volume: 228, anno: 1996,
pagine: 292 - 305
SICI:
0014-4827(1996)228:2<292:AOLCBE>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
TISSUE TRANSGLUTAMINASE; DNA FRAGMENTATION; POLY(ADP-RIBOSE) POLYMERASE; DEATH APOPTOSIS; FLOW-CYTOMETRY; INTERNUCLEOSOMAL FRAGMENTATION; MAMMALIAN-CELLS; HELA-CELLS; K562 CELLS; CYCLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
G. Bonelli et al., "APOPTOSIS OF L929 CELLS BY ETOPOSIDE - A QUANTITATIVE AND KINETIC APPROACH", Experimental cell research, 228(2), 1996, pp. 292-305

Abstract

Exponentially growing L929 cells were continuously exposed to 1 or 10mu M etoposide (VP-16). The effects of such treatment on cell growth,cycle distribution, morphology, and selected biochemical events were examined, DNA synthesis rates were markedly decreased and the protein/DNA ratio increased (unbalanced growth). Growth was blocked, with mostcells being cycle arrested by 24 h in (late S-)G2-M. An asynchronous process of cell death then developed. Cells initially shrank into eosinophilic, trypan blue-excluding bodies, which were then released into the medium, and eventually became permeable to trypan blue. Transmission electron microscopy confirmed that dying cells acquired an apoptotic morphotype, with compaction and margination of chromatin, loss of microvilli, and shrinkage of cytoplasm and nucleus, Tissue transglutaminase activity and intensity of immunostaining rapidly increased in treated cultures. Internucleosomal DNA fragmentation could not be detectedby agarose gel electrophoresis, yet flow cytometry revealed that the apoptotic bodies had a very low DNA fluorescence (less than or equal to 10% of the 2n value), In agreement with the microscopic findings, this suggested that extensive DNA degradation had occurred in dead cells. While rates of cell loss from the monolayer amounted to 21 and 57% day(-1) (1 and 10 mu M VP-16, respectively), apoptotic indexes largely underestimated the extent of the process. These indexes only measured the accumulation of apoptotic bodies, i.e., the balance between their generation and disposal. The latter occurred by mechanisms similar to those that operate in tissues: ''secondary necrosis'' or phagocytosis by viable homotypic cells in the monolayer (''homophagy''). (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 17:12:28