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Titolo:
EFFECTS OF FUMONISIN B-1 AND (HYDROLYZED) FUMONISIN BACKBONE AP(1) ONMEMBRANES - A SPIN-LABEL STUDY
Autore:
YIN JJ; SMITH MJ; EPPLEY RM; TROY AL; PAGE SW; SPHON JA;
Indirizzi:
US FDA,CTR FOOD SAFETY & APPL NUTR,200 C ST SW WASHINGTON DC 20204
Titolo Testata:
Archives of biochemistry and biophysics
fascicolo: 1, volume: 335, anno: 1996,
pagine: 13 - 22
SICI:
0003-9861(1996)335:1<13:EOFBA(>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALTERS SPHINGOLIPID METABOLISM; ELECTRON DOUBLE-RESONANCE; VERTICAL FLUCTUATIONS; FUSARIUM-MONILIFORME; LATERAL DIFFUSION; CHAIN; BILAYER; PHOSPHOLIPIDS; CHOLESTEROL; TOXICITY;
Keywords:
FUMONISIN; MEMBRANE; ESR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
J.J. Yin et al., "EFFECTS OF FUMONISIN B-1 AND (HYDROLYZED) FUMONISIN BACKBONE AP(1) ONMEMBRANES - A SPIN-LABEL STUDY", Archives of biochemistry and biophysics, 335(1), 1996, pp. 13-22

Abstract

Electron spin resonance (ESR) spectroscopy and spin label techniques have been used to study the effects of fumonisin B-1 (FB1) and hydrolyzed fumonisin backbone (AP(1)) on the structural and dynamic properties of phosphatidylcholine membranes at the molecular level. Multilamellar liposomes consisting of dimyristoylphosphatidylcholine (DMPC) and egg yolk phosphatidylcholine (EYPC) were used. Six different nitroxide spin labels were used to determine what effects FB1 may impart on the ordering and mobility of lipids in membranes. The experimental resultsdisclose the following: (1) In the fluid phase membrane, FB1 significantly increases the fluidities of n-doxylstearic acid (SA) spin labels(SL) attached to carbons 5 and 7, which disorders the alkyl chains and perturbs the surface region of the bilayer; by comparison, minimal effects were detected near the center of the bilayer. (2) In the gel phase, FB1 and AP(1) imparts marked rigidifying effects on membrane fluidity, which enlarges the change in ordering on the phase transition even further. (3) FB1 also restricts the mobility of the (rigid) cholestane spin label. (4) A reduction in mobility of the tempo-stearate spinlabel suggests that the tricarballylic acid (TCA) moieties of FB1 might mimic the structure of polar headgroups in phospholipids. The present results may provide additional mechanisms to elucidate the toxicological activities of the fumonisins. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 16:28:09