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Titolo:
EXPRESSION AND REGULATION OF GLIAL-CELL-LINE-DERIVED NEUROTROPHIC FACTOR (GDNF) MESSENGER-RNA IN HUMAN ASTROCYTES IN-VITRO
Autore:
MORETTO G; WALKER DG; LANTERI P; TAIOLI F; ZAFFAGNINI S; XU RY; RIZZUTO N;
Indirizzi:
UNIV VERONA,POLICLIN BORGO ROMA,DIPARTIMENTO SCI NEUROL & VIS,SEZ NEUROL I-37134 VERONA ITALY UNIV BRITISH COLUMBIA,DEPT PSYCHIAT,KINSMEN LAB NEUROL RES VANCOUVER BC V5Z 1M9 CANADA UNIV VERONA,CLIN OSTET & GINECOL I-37100 VERONA ITALY AMGEN INC THOUSAND OAKS CA 91320
Titolo Testata:
Cell and tissue research
fascicolo: 2, volume: 286, anno: 1996,
pagine: 257 - 262
SICI:
0302-766X(1996)286:2<257:EAROGN>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; NECROSIS-FACTOR-ALPHA; MIDBRAIN DOPAMINERGIC-NEURONS; MESSENGER-RNA; MOTOR-NEURONS; IN-VIVO; RAT; SURVIVAL; GENE; PROLIFERATION;
Keywords:
ASTROCYTES; GDNF; HUMAN CELL CULTURES; MESSENGER-RNA; NEUROTROPHIC FACTORS; PROTEIN KINASE C; HUMAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
G. Moretto et al., "EXPRESSION AND REGULATION OF GLIAL-CELL-LINE-DERIVED NEUROTROPHIC FACTOR (GDNF) MESSENGER-RNA IN HUMAN ASTROCYTES IN-VITRO", Cell and tissue research, 286(2), 1996, pp. 257-262

Abstract

The expression and modulation of mRNA for glial-cell-line-derived neurotrophic factor (GDNF) in human glial cells was investigated. Astrocyte cell cultures were isolated from human fetal brains, characterized by immunocytochemistry and maintained in vitro in conditions of high purity; sister cultures were exposed to protein kinase C (PKC) inhibitors for 20 min. Total RNA was extracted from the cell pellets, reverse-transcribed into cDNA and amplified by the polymerase chain reaction (PCR) with primers specific for GDNF. A reverse-transcription/PCR procedure was also performed on mRNA extracted from human fibroblast and lymphocyte cell lines. Human astrocytes grown in the absence of neurons expressed detectable amounts of mRNA for GDNF but no amplification products were observed in fibroblasts and lymphocytes, thus confirming that GDNF production was cell-type specific. After exposure to PKC inhibitors, a dramatic down-regulation of GDNF mRNA was observed in astrocyte cell cultures, Thus, human astrocytes are constitutively capable ofproducing GDNF, such trophic activity is restricted to neural cells, and PKC plays key roles in signal pathways that regulate the gene activation and production of GDNF.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 11:12:51