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Titolo:
EXPRESSION OF CADHERIN-CATENIN COMPLEXES IN HUMAN LEUKEMIA-CELL LINES
Autore:
TSUTSUI J; MORIYAMA M; ARIMA N; OHTSUBO H; TANAKA H; OZAWA M;
Indirizzi:
KAGOSHIMA UNIV,FAC MED,DEPT BIOCHEM,8-35-1 SAKURAGAOKA KAGOSHIMA 890 JAPAN KAGOSHIMA UNIV,FAC MED,DEPT BIOCHEM KAGOSHIMA 890 JAPAN KAGOSHIMA UNIV,FAC MED,DEPT MED 1 KAGOSHIMA 890 JAPAN
Titolo Testata:
Journal of Biochemistry
fascicolo: 5, volume: 120, anno: 1996,
pagine: 1034 - 1039
SICI:
0021-924X(1996)120:5<1034:EOCCIH>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULE E-CADHERIN; ADHESION MOLECULE; N-CADHERIN; ALPHA-CATENIN; BETA-CATENIN; CYTOPLASMIC DOMAIN; EPITHELIAL-CELLS; BREAST-CARCINOMA; BINDING PROTEINS; APC PROTEIN;
Keywords:
ATL; N-CADHERIN; ALPHA-CATENIN; BETA-CATENIN; LEUKEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
J. Tsutsui et al., "EXPRESSION OF CADHERIN-CATENIN COMPLEXES IN HUMAN LEUKEMIA-CELL LINES", Journal of Biochemistry, 120(5), 1996, pp. 1034-1039

Abstract

Cadherins are Ca2+-dependent cell-cell adhesion molecules, and are involved in the formation and maintenance of the histo-architecture. Using cultured human leukemia cell lines (adult T cell leukemia and thymus-derived lymphoma cell lines), we obtained evidence that cadherins and catenins are expressed in these cell lines but not in normal leukocytes, Immunoblot analysis of cells using a pan-cadherin serum, directedagainst the conserved carboxyl-terminus of cadherins, revealed a major band of 130 kDa and a minor one of 135 kDa, The 130 kDa cadherin wasalso recognized by anti-N-cadherin antibodies, A human N-cadherin cDNA probe hybridized to a 4.3 kb mRNA isolated from cells immunologically positive for N-cadherin, Sequencing of the cDNA fragments isolated from the cells revealed a N-cadherin sequence, Cell surface expression of N-cadherin was confirmed by indirect immunofluorescence staining ofthe cells, Immunoblot and Northern blot analyses also revealed the presence of alpha-catenin, beta-catenin, and gamma-catenin (plakoglobin)in these cell lines, Immunoprecipitation with anti-N-cadherin antibodies and subsequent immunoblot analysis with anti-catenin antibodies revealed that N-cadherin is associated with alpha- and beta-catenins, a prerequisite for cadherins to be functional, These results suggest an important role of the cadherin-catenin complexes in the behavior of the leukemia cells.

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Documento generato il 09/07/20 alle ore 00:30:58