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Titolo:
FIBRONECTIN PROMOTES THE PROLIFERATION OF CYTOTOXIC T-LYMPHOCYTES GENERATED FROM CANCER-PATIENTS
Autore:
MIZOBATA S; TANIMURA H; YAMAUE H; TANI M; TSUNODA T; IWAHASHI M; NOGUCHI K; NISHIMOTO N; HOTTA T; ARII K;
Indirizzi:
WAKAYAMA MED COLL,DEPT SURG 2,27 SHICHIBANCHO WAKAYAMA 640 JAPAN WAKAYAMA MED COLL,DEPT SURG 2 WAKAYAMA 640 JAPAN
Titolo Testata:
British Journal of Cancer
fascicolo: 10, volume: 74, anno: 1996,
pagine: 1598 - 1604
SICI:
0007-0920(1996)74:10<1598:FPTPOC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBSEQUENTLY CHALLENGED INVITRO; STREPTOCOCCAL PREPARATION OK432; TUMOR-INFILTRATING LYMPHOCYTES; NATURAL-KILLER CELLS; AUTOLOGOUS TUMOR; METASTATIC MELANOMA; PRIMED INVIVO; CD4 CELLS; INTERLEUKIN-2; VLA-5;
Keywords:
FIBRONECTIN; CYTOTOXIC T LYMPHOCYTE; ANTI-CD3 MONOCLONAL ANTIBODY; ADOPTIVE IMMUNOTHERAPY; INTERLEUKIN 2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
S. Mizobata et al., "FIBRONECTIN PROMOTES THE PROLIFERATION OF CYTOTOXIC T-LYMPHOCYTES GENERATED FROM CANCER-PATIENTS", British Journal of Cancer, 74(10), 1996, pp. 1598-1604

Abstract

We studied whether fibronectin (FN) enhances the activity of autologous tumour-reactive cytotoxic T lymphocytes (CTLs) generated from cancer patients. The proliferation of CTLs stimulated by immobilised anti-CD3 monoclonal antibody and interleukin 2 (IL-2) was enhanced three or four times by immobilised FN, whereas soluble FN did not alter the DNAsynthesis of CTLs. Moreover, the cytotoxic activity of CTLs was augmented by FN stimulation against autologous tumour cells [4 h Cr-51 release assay: FN(+) 16.7+/-4.7% vs FN(-) 11.8 +/- 3.1%; 16 h Cr-51 release assay: FN(+) 24.8 +/- 4.7% Fs FN(-) 16.5 +/- 5.7%, P<0.05]. The major cell surface phenotype of CTLs with FN was CD3(+), CD4(+) and CD25(+) in 6 weeks' culture. Cytotoxicity against autologous tumour cells was inhibited by anti-HLA class I monoclonal antibody (MAb). The autologous tumour-killing activity of CTLs was suppressed by the elimination of CD4(+) cells. Moreover, the cytokine production of CTLs was augmented by FN stimulation. Especially the production of IL-2, interferon gamma (IFN-gamma), and granulocyte-macrophage colony-stimulating factor (GM-CSF) was significantly augmented by FN stimulation (P<0.05). Thus,CTLs generated by FN might have both killer and helper Functions, since they could lyse autologous tumour cells and secrete various cytokines, including IL-2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 03:50:56