Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ALTERNATIVE RNA SPLICING OF THE MLL GENE IN NORMAL AND MALIGNANT-CELLS
Autore:
NAM DK; HONOKI K; YU M; YUNIS JJ;
Indirizzi:
911 SUMMIT RD NARBERTH PA 19072 THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT PATHOL ANAT & CELL BIOL,DIV CANC BIOL PHILADELPHIA PA 19107
Titolo Testata:
Gene
fascicolo: 1-2, volume: 178, anno: 1996,
pagine: 169 - 175
SICI:
0378-1119(1996)178:1-2<169:ARSOTM>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZINC-BINDING DOMAINS; ACUTE LEUKEMIAS; DROSOPHILA-TRITHORAX; SEQUENCE-ANALYSIS; BITHORAX COMPLEX; ALL-1 GENE; PROTEIN; EXPRESSION; DNA; TRANSLOCATIONS;
Keywords:
ALTERNATIVE RNA SPLICING; MLL PROTEIN MOTIFS; HOMEOBOX GENES; HEMATOPOIETIC CELLS; LEUKEMIA; RT-PCR; CLONING; DNA SEQUENCING; NORTHERN BLOT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
D.K. Nam et al., "ALTERNATIVE RNA SPLICING OF THE MLL GENE IN NORMAL AND MALIGNANT-CELLS", Gene, 178(1-2), 1996, pp. 169-175

Abstract

The human MLL(mixed-lineage leukemia or myeloid-lymphoid leukemia) gene belongs to the trithorax gene family of which the Drosophila trithorax (trx) gene is known to regulate homeotic genes through alternativeRNA splicing. To test if such a splicing mechanism also operates in MLL, we evaluated mRNA transcripts from a large number of normal and malignant human cells, making use of RT-PCR, PCR cloning, DNA sequencingand Northern blot analysis. Our findings indicate that different celltypes transcribe MLL mRNA species lacking exons that generally encodeputative regulatory domains such as AT hooks (exon 3), repression domain (exon 6), zinc finger motifs (exon 8) and activation domain (exon 18). Such findings suggest that posttranscriptional regulation by alternative RNA splicing may play an important role in MLL gene expressionand provides the rationale for a mechanism by which this gene, with multiple functional domains, could produce discrete protein products that may prove critical in the regulation of human homeobox genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 23:22:37